Identification of differentially expressed genes in human uterine leiomyomas using differential display  

作　　者：BIN LI, MEI SUN, BIN HE, JIN YU, You DUAN ZHANG, YONG LIAN ZHANG, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Science, Chinese Academy of Sciences, 820, Yue- Yang Road, Shanghai, 

出　　处：《Cell Research》2002年第1期39-45,共7页细胞研究（英文版）

摘　　要：In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis. RESULTS: Four of 69 cDNA fragments (3 up-regulated named L1, L2 and L3 and 1 down-regulated named M1 in leiomyoma) were confirmed by Northern analysis. Sequence comparison and Northern analysis proved that L1 is exactly the human ribosomal protein S19. It was present ubiquitously in 13 tissues tested but in various levels and even in different size. L1 was highly expressed in parotidean cystadenocarcinoma, pancreatic cancer and breast cancer examined. No mutations have been found in human uterine leiomyomas (n=6). CONCLUSIONS: hRPS19 overexpression might be a universal signal in rapid cell growth tissues.

关 键 词：Gene Expression Profiling Peptide Library Comparative Study DNA  Complementary Female Gene Expression Regulation  Neoplastic Humans LEIOMYOMA MYOMETRIUM NEOPLASMS Research Support  Non-U.S. Gov't Ribosomal Proteins Tissue Distribution Uterine Neoplasms 

分 类 号：Q987[生物学—遗传学]