尖锐湿疣病变的人乳头瘤病毒6型L1序列多态性分析  

Intratypic Nucleotide and Amino Acid Sequence Variations in the L1 Open Reading Frame of Human Papillomavirus Type 6 in Condyloma Acuminata Lesions

作　　者：洪少林[1] 王家璧[1] 李平川[2] 周玲[2] 司静懿[3] 许雪梅[3] 郭秀婵[2] 曾毅[2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院,北京100730 [2]中国疾病预防控制中心病毒病预防控制所,北京100052 [3]中国医学科学院中国协和医科大学基础医学研究所,北京100005

出　　处：《病毒学报》2002年第2期102-107,共6页Chinese Journal of Virology

基　　金：国家高技术研究发展计划 (2 0 0 1AA2 15 2 2 1) ;863计划 (2 0 0 1AA2 15 2 2 1)

摘　　要：采用PCR方法从协和医院尖锐湿疣患者皮损中检测人乳头瘤病毒 (HPV) ,并通过限制性片断长度多态性分析分型发现 ,HPV6型为主要感染型别 ,其次是HPV11型。根据临床特征选择主要致病型HPV6 8个分离株 ,扩增L1晚期基因 ,构建重组测序质粒 ,双脱氧法测序并分析L1区的核苷酸和氨基酸序列变异状况。结果表明 ,HPV6L1基因序列发生碱基替换的区域主要有四个区 ,包括SR1(5 911～ 6 10 4 ) ,SR2 (6 2 17～ 6 2 73) ,SR3(6 5 4 0～6 6 6 1)和SR4 (70 6 2～ 72 5 0 )。少数的碱基替换导致错义突变 ,推导的蛋白质一级结构中有 0～ 3个氨基酸发生变异 。PCR was used to determine the human papillomavirus (HPV) in genital wart lesions from patients in PUMC hospital.Type of HPV was identified by restriction fragment length polymorphism analysis The predominant type was HPV6,secondly the HPV11.Eight isolates of HPV6 with different clinical manifestations were chosen for further intratypic nucleotide and amino acid sequence variation analysis.HPV6 L1 genes of eight isolates were amplified and inserted into plamid pMD18-T.By sequencing and phylogenetic analysis,the most frequently observed substitutions in HPV6 L1 ORF were distributed in four discrete regions:SR1(5911～6104),SR2(6217～6273),SR3(6540～6661) and SR4(7062～7250).Some substitutions resulted in non-silent mutation.There had 0 to 3 deduced amino acid changes in different L1 sequences,but which didn't affect immunogenicity of L1 protein obviously.

关 键 词：尖锐湿疣病变 人乳头瘤病毒6型 L1序列多态性 聚合酶链式反应 L1开放读码框 基因序列多态性 

分 类 号：R373.9[医药卫生—病原生物学] R752.53[医药卫生—基础医学]