牛泡沫病毒长末端重复序列在大肠杆菌中的启动子功能  

Bovine Foamy Viral Long Terminal Repeat is Functional and Can Be Trans-activated in Escherichia coli

作　　者：郭春光[1] 乔文涛[1] 胡文治[1] 王金忠[1] 陈启民[1] 耿运琪[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《病毒学报》2002年第2期181-184,共4页Chinese Journal of Virology

基　　金：国家自然科学基金 (3 9970 0 3 3 ) ;教育部博士点专项基金资助项目 (980 0 5 5 16)

摘　　要：The long terminl repeat(LTR) of the bovine foamy virus(BFV) contains the viral promoter,which is responsible for viral gene expression in eukaryotic cells We have demonstrated that BFV LTR linked to the luciferase gene can express the enzyme efficiently upon transformation into bacteria Deletion analysis and sequence comparison showed that the BFV LTR has a sequence(from-125 to-90)which is greatly homologous to the model bacteria promoter And the proposed transcriptional starting site is at the thymine of-91 or the cytosine of -92 Besides,being fully functional in E coli,the BFV LTR can also be specifically trans activated by BFV tas gene product,Borf-1 protein The responsive element lies between base -310 and -140,which is in accordance with the responsive region in eukaryotic cells The trans-activation of BFV LTR by Borf 1 protein in bacteria offers a useful system to investigate further the specific interaction between Borf-1 protein with BFV LTR and the mechanism of the transThe long terminl repeat(LTR) of the bovine foamy virus(BFV) contains the viral promoter,which is responsible for viral gene expression in eukaryotic cells We have demonstrated that BFV LTR linked to the luciferase gene can express the enzyme efficiently upon transformation into bacteria Deletion analysis and sequence comparison showed that the BFV LTR has a sequence(from-125 to-90)which is greatly homologous to the model bacteria promoter And the proposed transcriptional starting site is at the thymine of-91 or the cytosine of -92 Besides,being fully functional in E coli,the BFV LTR can also be specifically trans activated by BFV tas gene product,Borf-1 protein The responsive element lies between base -310 and -140,which is in accordance with the responsive region in eukaryotic cells The trans-activation of BFV LTR by Borf 1 protein in bacteria offers a useful system to investigate further the specific interaction between Borf-1 protein with BFV LTR and the mechanism of the trans activation

关 键 词：牛泡沫病毒 长末端重复序列 大肠杆菌 启动子 功能 

分 类 号：S852.653[农业科学—基础兽医学] S852.659.3[农业科学—兽医学]