中国棉铃虫核多角体病毒基因组XbaI-I片段的序列分析  

Sequence Analysis of the Xba I-I Region of the Single Nucleocapsid Nucleopolyhedrovirus of Helicoverpa armigera

作　　者：冯枞棣[1] 陈新文[1] Just M.Vlak 胡志红[1] 

机构地区：[1]中国科学院武汉病毒研究所无脊椎动物病毒学联合实验室,湖北武汉430071 [2]Department of Virology,Wageningen University

出　　处：《中国病毒学》2002年第2期125-131,共7页Virologica Sinica

基　　金：国家转基因植物专项资助 (J0 0 -A -0 0 3);中国科学院创新工程重要方向项目资助 (KSCX2 -1-0 2 )

摘　　要：报道了棉铃虫单核衣壳核多角体病毒 (HaSNPV)XbaI I片段的序列结构。该片段在基因组上定位于 18.4～2 2 .8m .u .,包括 8个完整的开放阅读框架和 p47的 5′端部分序列 :其中ubiquitin ,39K/pp31,lef - 11,p47,Ac34 ,Ac38和Lsel2 5homologue 7个基因是杆状病毒的同源基因 ,另外两个orf5 0 7和orf10 80是HaSNPV的特有基因 ,且具有典型的早期表达基因启动子的特征 ,经软件分析发现这两个基因推导的产物具有典型的跨膜压结构。序列比较分析表明 ,ubiquitin基因与其它杆状病毒的同源基因有相同的保守区 。The Xba I-I fragment located at 18.4～22.8 m.u. of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus genome was sequenced. The fragment contains eight complete ORFs, including putative ubiquitin, 39K/pp31, lef-11, Lsel25, orf507, orf1080, Ac34,Ac38 and a partial orf which potentially encodes C-terminal of P47. orf507 and orf1080 are two putative unique genes of HaSNPV, both contain an early-expression gene motif CAGT and a TATA box in upstream sequences. The ubiquitin gene of HaSNPV shows high identity with baculoviruses and human or yeast ubiquitins. The 39K/pp31 promotor of HaSNPV does not contain late promotor motifs, which exists in other so far identified baculoviral homologues, suggesting it probably has different transcriptional pattern.

关 键 词：中国 棉铃虫核多角体病毒 基因组XbaI-I片段 序列分析 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治] S435.622.3[农业科学—植物保护]