江苏水稻黑条矮缩病毒S10的cDNA克隆序列分析  

cDNA Clone and Sequence Analysis of Segment 10 of RiceBlack-Streaked Dwarf Fijivirus in Jiangsu

作　　者：王朝辉[1] 周益军[1] 范永坚[1] 程兆榜[1] 张文荟[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014

出　　处：《中国病毒学》2002年第2期142-144,共3页Virologica Sinica

基　　金：江苏省九五重点攻关 (BE96 386 );江苏省自然科学基金 (BJ97132 )

摘　　要：从来自江苏连云港并在本实验室保存的水稻黑条矮缩病毒接种的病株玉米中提取dsRNA ,采用改进的单引物扩增技术获得了病毒基因组片段S10的cDNA克隆并测定了其全序列。结果表明S10全长 180 1bp ,含有一个ORF ,组织结构与日本报道的RBSDV基本一致 ,核苷酸和推导的氨基酸序列与MRDV的相似性分别为 87.5 %和92 .6 % ,与RBSDV的相似性分别为 93.3%和 96 .4%。An isolate of rice black-streaked dwarf Fijivirus originally from Lianyuangng,Jiangsu,China was used to inoculated maize to propagate the virus.Improved single primer amplification technique was adopted to clone the viral genomic dsRNA S10 for sequence determination.The result showed:the full length of S10 is 1 801 bp and has the same organization with MRDV in Italy and RBSDV in Japan.The similarity of nucleotide and predicted protein were 87.5% and 92.6% with MRDV,\{93.3%\} and 96.4% with RBSDV.This study provides a new method for the sequence determination of viral dsRNA.

关 键 词：江苏 水稻黑条矮缩病毒S10 CDNA克隆 序列分析 

分 类 号：S432.4[农业科学—植物病理学]