草鱼呼肠孤病毒新分离株(GCRV_(991))的病毒学特性分析  

Virological Properties of GCRV_(991) Strain

作　　者：方勤[1] 肖调义[2] 丁清泉[3] 李旅[3] 章怀云[2] 朱作言[1] 

机构地区：[1]淡水生态与生物技术国家重点实验室,中国科学院水生生物研究所,武汉43072 [2]湖南农业大学水产学院,长沙410128 [3]中国科学院武汉病毒研究所,武汉430071

出　　处：《中国病毒学》2002年第2期179-181,共3页Virologica Sinica

基　　金：国家自然科学基金资助项目 ( 30 170 730 );国家"86 3"高科技攻关项目 ( 10 1 0 5 0 5 0 4) ;淡水生态与生物技术国家重点实验室开放基金 ( 2 0 0 2FB0 5 )

摘　　要：从湖南长沙分离到一株致病性强的草鱼呼肠孤病毒 (GCRV991) ,该病毒能使草鱼CIK ,肥头鲤FHM细胞产生明显的CPE ,对水生动物BF2 ,EPC及哺乳动物BHK ,VERO细胞株不敏感。中和实验显示 ,GCRV873 抗体能有效地中和GCRV991病毒颗粒 ,形成抗原抗体免疫复合物。纯化的病毒核酸与蛋白经SDS PAGE分离 ,分别呈现 11条清晰的核酸带及 5条主要与 2条微量结构多肽图谱 ,其核酸蛋白分子量大小与GCRV873 相近似。该毒株基因组总分子量为 14.48× 10 6kD ,大小范围是 0 .5 5～ 2 .6 1× 10 6kD ;5条主要与两条微量结构多肽分子量近似值分别为136kD、132kD、6 5kD、43kD、34kD及 138kD、82kD。上述结果提示 ,新分离的GCRV991与GCRV873A powerful pathogenic Grass Carp reovirus (GCRV\-\{991\}) was recently isolatered from Changsha, Hunan provice of China. The virus replicated well in CIK,FHM cell line, but did not infect another 2 aquatic BF2, EPC and 2 mammalian BHK, Vero cell lines. It showed that the virus could be neutrialized by GCRV\-\{873\} antibody, and also the immunocompound was detected by electronic microscope. polyacrylamide gel electrophoesis revealed that GCRV\-\{991\} genome was composed of 11 segments of dsRNA, and its structural protein was consisted of 5 major plus 2 minor structural polypeptieds, which both of the properties were also shared by GCRV\-\{873\}. GCRV\-\{991\} total genome molecular weight is 14.48×10\+6kD, the rang is from 0.55-2.61×10\+6kD. The estimated 5 major and 2 minor structural protein values were 136,132,65,43,34kD, and 138,82kD, respectively. It is suggested the new isolate GCRV\-\{991\} may show same antigen properties with GCRV\-\{873\}.

关 键 词：草鱼呼肠孤病毒 分离株 GCRV991 病毒学特性 

分 类 号：S943[农业科学—水产养殖]