四株草鱼呼肠孤病毒毒株的细胞感染特性比较研究  

Infection Characterizations of four Grass Carp Reovirus(GCRV) Strains

作　　者：方勤[1] 肖调义[2] 李旅[3] 邹桂平[3] 章怀云[2] 汪亚平[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]湖南农业大学水产学院,长沙410128 [3]中国科学院武汉病毒研究所,武汉430071

出　　处：《中国病毒学》2002年第2期182-184,共3页Virologica Sinica

基　　金：国家自然科学基金资助项目 ( 310 70 730 );国家"86 3"高科技攻关项目 ( 10 10 5 0 5 0 4);淡水生态与生物技术国家重点实验室开放基金 ( 2 0 0 2FB0 5 )

摘　　要：本文首次对低温保存的三株草鱼呼肠孤病毒GCRV873 、GCRV875、GCRV876与新分离的GCRV991毒株进行了细胞培养与病毒感染特性等比较研究。结果表明 ,GCRV873 、GCRV875、GCRV876在 - 30℃保存 10年后仍然具有一定的感染性 ,其滴度均在 10 2 TCID50 /mL以上 ,略低于从病鱼组织分离的GCRV991毒株的滴价。经传代培养后 ,四株GCRV的毒力逐渐升高 ,并趋于稳定 ;当感染复数 (MOI)为 0 .0 5PFU/cell时 ,测定四株GCRV的滴度均高于 10 8TCID50 /mL ,但略有差异。GCRV873 的滴度最高 ,可达到 6 .4× 10 11TCID50 /mL。连续传代的GCRV毒株在不同温度 (2 8℃、31℃、34℃、37℃、41℃ )条件下 ,均可感染CIK细胞 ;在 2 8℃时 ,感染效价最高 ,随着温度的升高 。Some characterizations of four Grass Carp Reovirus (GCRV) strains were comparatively studied at the first time. It showed that the stored GCRV\-\{873\},GCRV\-\{875\} and GCRV\-\{876\} at minus 30℃ for ten years still remained infectious to CIK cells, and the 3 virus titers could reach above 10\+2 TCID\-\{50\}/mL, which was a little lower than the newly isolated GCRV\-\{991\} strain′s. After inoculation 3-4 generations, their titers rised gradually, and the titer of GCRV\-\{873\} could be up to the highest, which was almost 6.4×10\+\{11\} TCID\-\{50\}/mL with 0.05PFU/cell multiplicity of infection (MOI) at 28℃. The infective ability of the four strains at different temperatures (28℃,31℃,34℃,37℃ and 41℃) was also detected, which revealed that CPE appeared in CIK cells under above conditions, but titers depressed as the temperature rised. In addition, 4 GCRV-ds RNAs were also compared in a same electronic condition.

关 键 词：草鱼呼肠孤病毒毒株 细胞感染特性 比较研究 

分 类 号：S943[农业科学—水产养殖]