高压氧治疗对新生大鼠缺氧缺血性脑损伤后神经肽Y_(1-36)和降钙素基因相关肽含量的影响  

The effects of hyperbaric oxygen therapy of hypoxicischemic brain injury on levels of neuropeptide Y_(1-36) and calcitonin gene-related peptide in neonatal rats

作　　者：卢晓欣[1] 洪新如[2] 汤永建[1] 何苏育[1] 

机构地区：[1]南京军区福州总医院高压氧科,福州350025 [2]南京军区福州总医院儿科,福州350025

出　　处：《中华航海医学与高气压医学杂志》2002年第2期82-85,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：全军"九五"医学科研规划基金资助课题 (98D0 2 2 )

摘　　要：目的　观察高压氧 (HBO)治疗新生大鼠缺氧缺血性脑损伤后血浆和大脑皮层的神经肽Y1 - 36 (NPY)和降钙素基因相关肽 (CGRP)含量的动态变化。方法　 7d龄新生大鼠左侧颈总动脉结扎形成缺血 ,并吸低氧氮混合气形成缺氧 ,随机分为脑缺氧缺血 +常氧高压氮组、脑缺氧缺血 +HBO治疗组、假手术组和正常对照组 ,再按不同时间各分成 4个小组。新生大鼠缺氧缺血性脑损伤后即刻行 HBO治疗 ,用放射免疫分析法测定治疗后不同时间血浆和大脑皮层的神经肽 Y1 - 36 和降钙素基因相关肽免疫活性物质 (ir- NPY、ir- CGRP)含量的变化。结果　缺氧缺血性脑损伤后不同时间 ,血浆 ir- NPY和 ir-CGRP、双侧皮层 ir- NPY、伤侧皮层 ir- CGRP含量不同程度升高 ;HBO治疗 ,可部分抑制血浆 ir- NPY、ir- CGRP的升高 ,左侧皮层 ir- NPY水平的升高被明显抑制 ,右侧皮层 ir- CGRP水平受影响较小。结论　缺氧缺血性脑损伤的发生发展与外周及中枢神经肽 Y1 - 36 和降钙素基因相关肽的过量生成有关。HBO治疗降低外周神经肽 Y1 - 36 和降钙素基因相关肽 ,降低伤侧脑区神经肽 Y1 - 36 的水平 。Objective To investigate the peripheral and central dynamic changes of levels of neuropeptide Y 1 36 and calcitonin gene related peptide after hyperbaric oxygen therapy(HBO) of hypoxic ischemic brain injury(HIBI) in neonatal rats. Methods Seven day old Sprague Dawley rats were randomly divided into four groups:HIBI+normoxic hyperbaric nitrogen group, HIBI+HBO group, sham operated group and normal control group with each group divided into four subgroups according to the time schedule. Neonatal HIBI model was preformed by ligating the left common carotid artery and then by breathing in a 37℃ humidified 8% oxygen and balanced nitrogen at 1.5 L/min for 2.5 h. Changes of levels of both neuropeptide Y 1 36 like and calcitonin gene related peptide like immunoreactives(ir NPY and ir CGRP) in plasma and cortices were tested by radioimmunoassay 0,12,24 and 48 h after HBO treatment. Results Significantly elevated levels of ir NPY and ir CGRP in plasma, ir NPY in left and right cortices, ir CGRP in left cortex were observed at different time intervals after HIBI. High levels of both ir NPY and ir CGRP in plasma were partially attenuated, and high levels of ir NPY in left cortex were significantly reversed with the HBO treatment. Little effect of HBO on levels of ir CGRP of cortex after HIBI was seen. Conclusions Pathophysiological process of HIBI might be related to the peripheral and central excessive secretion of neuropeptide Y 1 36 and calcitonin gene related peptide. One of the pathways of alleviating HIBI by HBO might be the inhibition of these high levels of ir NPY and ir CGRP in plasma and of ir NPY in cortex ipsilateral to the damage.

关 键 词：神经肽Y-36 降钙素基因相关肽 脑缺血 脑缺氧 新生儿 缺氧缺血性脑病 高压氧治疗 

分 类 号：R459.6[医药卫生—治疗学]