肥大细胞在单纯和放射复合伤口愈合中的作用  

Role of mast cell and its subpopulations in simple and irradiated-wound of rats

作　　者：崔玉芳[1] 夏国伟[1] 付小兵[2] 杨红[1] 彭瑞云[1] 高亚兵[1] 崔雪梅[1] 王德文[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]解放军第304医院创伤研究中心,北京100037

出　　处：《中国病理生理杂志》2002年第5期458-461,共4页Chinese Journal of Pathophysiology

基　　金：由国家"973"项目资助 (G19990 5 4 2 0 4 )

摘　　要：目的 :观察肥大细胞 (MC)及其亚群在单纯和放射复合伤口中的动态变化 ,旨在探讨MC在其中的作用及其机制。方法 :应用阿里新兰 -番红花红 (ABS)双染色法 ,检测MC及其亚群。结果 :①伤后 2d两组MC总数均出现一致降低 ,伤后 5 - 7dMC总数迅速升高并逐渐达到峰值 ,且一直维持到伤后 15 - 2 8d。②粘膜型MC(MMC)和结缔组织型MC(CTMC)亚群也出现规律性变化。伤后 2d ,MMC和混合型MC(MixMC)均出现明显降低 ,此后一直维持在较低水平。而CTMC伤后一直维持在较高水平。③伤照组MixMC在伤后 5d、MC总数在伤后 5 - 15d ,均明显少于单伤组。结论 :MC及其亚群在创伤愈合以及辐射延迟伤口愈合过程中起着重要作用。AIM:To examine the role and dynamic changes of mast cell(MC) and its subpopulations in simple and irradiated-wound of rats.METHODS:MC and its subpopulations were estimated using alcian blue-safranin (ABS)double staining RESULTS:(1)The total number of MC in two groups decreased coincidently on day 2 after wounding, and the total MC increased rapidly and reached maximal gradually on day 5 and 7 after wounding, the increment of MC remained consistently 28 day after wounding (2)Both mucosal MC( MMC) and Mix MC decreased obviously on day 2 after wounding, hereafter,they remained the low level all the time However, the CTMC kept in the high level after wounding (3)The Mix MC on day 5 and the total MC during day 5-15 after wounding were lower in irradiated group than in simple wound group CONCLUSION: MC and its subpopulations could delay the healing process of simple and irradiated wound

关 键 词：伤口愈合 辐射 阿里新兰-番红花红双染色法 肥大细胞 

分 类 号：R392.3[医药卫生—免疫学]