抑制大鼠动脉损伤后早期中膜平滑肌细胞增殖可降低内膜肥厚  

Reduction of intimal thickening by inhibition of early rat medial smooth muscle cell proliferation induced by arterial injury

作　　者：舒强[1] 石恩金[1] 蒋健[2] 凌光烈[1] 

机构地区：[1]中国医科大学附属第一医院外科局解教研室,辽宁沈阳110001 [2]上海中医药大学附属曙光医院内科,上海200021

出　　处：《中国病理生理杂志》2002年第5期462-465,共4页Chinese Journal of Pathophysiology

基　　金：留学归国人员科研启动基金资助项目 (1999- 74 7)

摘　　要：目的 :探讨早期中膜平滑肌细胞增殖与内膜肥厚的关系。方法 :球囊导管损伤大鼠动脉后 ,在不同时期投予血管紧张素转换酶抑制剂 (ACEI) (temocapril-HCl,10mg·kg-1·d-1) ,观察平滑肌细胞BrdU(5 -溴脱氧尿嘧啶尿苷 )标记率和内膜面积。结果 :损伤后动脉中膜平滑肌BrdU阳性细胞出现于术后 2 4h ,BrdU标记率呈双峰期变化 ,第 1期在 1- 3d ,峰值在 2d为 5 3% ,第 2期在 3- 7d ,峰值在 5d为 2 7%。在第 1个增殖期投予temocapril显著抑制第 2个增殖期的BrdU标记率 (0 0 5± 0 0 2 ) % ,对照组为 (4 5 0± 0 2 7) % (P <0 0 1) ,同时也显著抑制了术后10d内膜面积的增加 (10 6 70 1μm2 ± 7713 3μm2 ) (P <0 0 1)。而仅在第 2个增殖期投药 ,则不能抑制内膜面积的增加 (83499 5 μm2 ± 3136 0 0 μm2 ) (P >0 0 5 ) ,其程度与未投药组相当。结论AIM: To investigate the relationship between medial smooth muscle cell proliferation and intimal thickening.METHODS: Angiotensin converting enzyme inhibitor (temocapril-HCl, 10 mg·kg -1 ·d -1 ) was administered in various periods after rat arterial injury by balloon catheters. The labeling BrdU indexes of smooth muscle cell and intimal areas were observed. RESULTS: Positive medial smooth muscle cells for BrdU occurred at 24 hours after injury. The labeling indexes for BrdU showed biphasic change, the period of the first peak appeared within day 1-3, the peak value was 5 3% at 2 days. The period of the second peak appeared within day 3-7, the peak was 2 7% at 5 days. Administration of temocapril in the first proliferation phase significantly suppressed the labeling BrdU index(0 05±0 02)% vs (4 50±0 27)%, P<0 01 in the second one, also the intimal area at 10 days [(10 670.1±7 713.3) μm 2, P<0 01 ]. Administration of temocapril in the second phase, however, did not effectively suppress the intimal formation [(83 499 5±31 360 0) μm 2] which was approximate to the vehicle-treated group( P>0 05 ). CONCLUSION: The first proliferative phase of arterial medial smooth muscle cell after balloon injury is necessary to the intimal formation.

关 键 词：大鼠 动脉损伤 中膜平滑肌细胞 内膜肥厚 血管紧张素转换酶抑制药 细胞增殖 

分 类 号：R543[医药卫生—心血管疾病]