冻干同种异体骨应力适应性的组织形态计量学和分子生物学研究  

A HISTOMORPHOMETRIC AND MOLECULAR STUDY ON THE STRESS ADAPTABILITY OF FREEZE-DRIED BONE ALLOGRAFT

作　　者：汤亭亭[1] 戴尅戎[1] 朱乃硕[2] 陈永强[1] 水汶[1] 

机构地区：[1]上海第二医科大学附属第九人民医院骨科,上海200011 [2]复旦大学生命学院病毒研究室,上海200433

出　　处：《医用生物力学》2002年第2期70-74,共5页Journal of Medical Biomechanics

基　　金：上海市教委科技发展基金资助

摘　　要：目的　探讨冻干同种异体骨的应力适应性。方法　 5 6只兔双前肢尺骨中段分别进行同种异体皮质骨和松质骨移植 ,并利用一简易外固定支架造成左侧移植骨承受的正常生理载荷和右侧承受的低载荷 ,分别在术后 2 ,4 ,8,1 6周处死取材 ,对植骨区标本进行骨形态计量学检查和胶原基因表达的原位杂交分析。结果骨移植后第 4 ,8,1 6周 ,正常载荷侧界面间的四环素标记表面和骨矿化沉积率都明显高于低载荷侧。反映移植骨内部新骨爬行替代的参数 ,包括皮质骨和松质骨内部的标记表面、皮质骨内部的矿化沉积率 ,到第 1 6周 ,正常载荷侧都明显高于低载荷侧。原位杂交结果发现 ,在界面间或移植骨内部的吸收陷窝内 ,正常载荷侧I型胶原基因表达比低载荷侧更为明显。结论　正常生理载荷的刺激有利于早期宿主骨 -冻干异体骨界面间的连接和异体骨内部新骨的爬行替代。Objective To Investigate the stress adaptability of freeze-dried bone allograft. Methods Cortical and cancellous allograft were transplanted to each side of the midshaft diaphyseal ulnar respectively in each group of 28 animals. The left transplanted allograft was free from fixaton and bear normal physiological load, while the right transplanted allograft was protected from loading by a simple external fixator and bear less load. Animals were killed in batch at 2nd, 4th, 8th, 16th week after transplantation and specimens were taken out for bone histomorphometry study and analysis of collagen gene expression by in situ cDNA-mRNA hybridization.Results Labeled surface(LS) and bone mineral apposition rate(MAR) of the normally loaded graft-host bone interface were significantly higher than that of the less loaded side at the 4th,8th,16th week after transplantation. The parameters reflecting the internal repair process of allograft such as LS in cortical and cancellous boen or MAR in cortical bone of the normally loaded side were significantly higher than that of the less loaded side at 16th week after transplantation. The result of in situ hybridization indicated that more osteoblast-like cells with expression of typeⅠcollagen gene occuring in the interface or interior of normally loaded graft. Conclusion The stimulus of physiologic load can accelerate the early union of allograft-host bone interface and later new bone creeping substitution to the necrotic allograft.

关 键 词：冻干同种异体骨 应力适应性 组织形态计量学 分子生物学 同种异体骨移植 基因 胶原 

分 类 号：R687.34[医药卫生—骨科学] R318.01[医药卫生—外科学]