八正合剂抗感染作用的实验研究  

Experimental study on anti-infection of BAZHENG MIXTURE

作　　者：吴捷[1] 曹舫 刘传镐[1] 杨银京[1] 刘如意[3] 

机构地区：[1]西安交通大学医学院药理学教研室,陕西西安710061 [2]陕西省肿瘤医院,陕西西安710061 [3]西安交通大学医学院微生物学教研室,陕西西安710061

出　　处：《中草药》2002年第6期523-525,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目 (No.3 9870 3 3 4)

摘　　要：目的　探讨八正合剂治疗泌尿系统感染性疾病的药理学作用基础。方法　将大肠杆菌注入小鼠膀胱后观察药物对细菌上行感染肾脏的抑制作用 ;家兔 iv大肠杆菌内毒素后观察药物对细菌内毒素引起机体发热的影响 ;采用大鼠足跖 sc角叉菜胶所致炎性肿胀模型观察药物对炎性肿胀及机械压迫足跖疼痛的抑制作用。结果　八正合剂 ig给药可显著降低大肠杆菌上行感染肾脏的带菌剖面百分率 [ED50 为 (11.0 1± 1.6 3) g/ kg,95 %可信限为9.5 0～ 12 .76 g/ kg],抑制大肠杆菌内毒素诱发家兔体温升高 ,减轻角叉菜胶所致大鼠足跖炎性肿胀及致炎后肿胀部位机械压迫痛阈降低程度。结论　八正合剂治疗泌尿系统感染性疾病的作用机制主要与其清除尿路细菌和减轻发热、肿胀、疼痛等炎症反应症状有关。Object To explore the pharmacological basis of BAZHENG MIXTURE (BM) on treating infectious diseases of urinary system. Methods The kidneys were examined for the anti infectious action of BM on retrograde infection after injecting Escherichia coli into urocysts of mice. Rabbits iv with the endotoxin of E. coli were used to observe the effects of BM on the fever induced by bacterial endotoxin. Also in this experiment, the inflammatory edema model induced by carrageenan in rat was used to test the inhibition of BM on both the inflammatory edema and the pain provoked by pressuring foot mechanically. Results BM ig remarkably decreased the percentage of kedney section carrier caused by retrograde infection of E. coil [ED 50 is (11.01±1.63) g/kg and the 95% CL is 9.50 12.76 g/kg]. BM was also shown to be effective in inhibiting the rise of body temperature induced by endotoxin of E. coli. In addition, BM reduced the inflammatory edema caused by carrageenan as well as the threshold decline of pressuring pain. Conclusion The mechanism of BM on the treatment of urinary infective diseases are mainly associated with clearing bacteria and alleviating inflammatory symptoms such as fever, edema and pain, etc.

关 键 词：八正合剂 抗感染作用 实验研究 中药 抗炎 解热 镇痛 

分 类 号：R285.5[医药卫生—中药学]