大白菜地方种质种内遗传关系的RAPD分析  

STUDIES ON GENETIC RELATIONSHIPS AMONG LOCAL GERMPLASM OF CHINESE CABBAGE BY RAPD

作　　者：黄宝勇[1] 曲士松[1] 孙晋斌[1] 张杰[1] 徐培文[1] 刘恒英[1] 

机构地区：[1]山东省农业科学院蔬菜研究所,济南250100

出　　处：《山东农业大学学报（自然科学版）》2002年第2期154-157,共4页Journal of Shandong Agricultural University：Natural Science Edition

摘　　要：对大白菜亚种内 11个地方种质材料 ,用改进的CTAB法提取DNA后进行RAPD(RandomAmplifiedPolymorphicDNA)分析。聚类结果表明 :当遗传距离为 0 .18时 ,11份材料可分为二个生态类群 ,第一类群属于直筒类型 (交叉性气候生态型 ) ,另一类群在 0 .15时 ,又分为二个类群 ,矮桩类型 (海洋性气候生态型 )和直筒与矮桩的过渡类型。同时表明 ,应用RAPD选择特定引物在同一亚种内不同品种间进行遗传多样性、亲缘关系及品种鉴定等研究是可行的。RAPD was used to evaluate polymorphism and genetic relationship of 11 local germplasm of Chinese Cabbage.In the experiment 11 materials were divided into two ecological groups when genetic distance was 0.18,one was Crossed Climate Ecotype and the other was also divided into two groups when the distance was 0.15,one Ocean Climate Ecotype and the other was transitional form.Meanwhile,studies of genetic relationship and polymorphism by RAPD with specific primer within subspecies were feasible.

关 键 词：大白菜 地方种质 种内遗传 RAPD 遗传关系 聚类分析 

分 类 号：S634.1[农业科学—蔬菜学]