ABO血型基因分型及应用  

Genotyping of ABO blood group and its application

作　　者：兰炯采[1] 孟庆宝[1] 张印则[1] 周华发[1] 王从容[1] 曹琼[1] 刘忠 

机构地区：[1]第一军医大学南方医院输血科,广州510515 [2]合肥血液中心,合肥230022

出　　处：《中国免疫学杂志》2002年第6期430-433,共4页Chinese Journal of Immunology

摘　　要：目的 :研究ABO血型基因分型的意义。方法 :采用聚合酶链反应 序列特异性引物 (PCR SSP)基因定型方法对ABO血型基因定型并观察其基因多态性分布特征和疑难血型检定。结果 :对已知ABO基因的DNA标本进行基因定型 ,证实文中的ABO基因定型方法可靠 ;对 10 4例健康、无血源关系的汉族个体ABO血型基因定型 ,结果与血清学所定表型完全符合 ;并用ABO基因分型技术解决临床输血前血型鉴定、产前胎儿血型鉴定、亲权试验及血清学亚型的正确性验证。结论Objective:To study on significance of ABO genotyping.Methods:To use the methods of polymerase chain reaction sequence specific primers(PCR SSP) for genotyping of ABO blood group and observation ABO gene polymorphism as well as identification of doubt sample.Results:The reliability of the genotyping for ABO blood group was proved by testing DNA samples previously known genotypes.The results of genotyping for 104 healthy and unrelated HAN individuals were correspond with serological phenotypes.The method of ABO genotyping was applied for clinical pre transfusion ABO typing,pre delivery fetal ABO typing,parenting test and subgrouptyping.Conclusion:The method of ABO genotyping may correct typing for doubt sample of ABO serological typing.

关 键 词：ABO血型 聚合酶链反应-序列特异性引物 PCR-SSP 基因分型 

分 类 号：R457.11[医药卫生—治疗学]