抗猪口蹄疫病毒单克隆抗体的研制及其生物学特性鉴定  

Development and biological characteristics of monoclonal antibodies against foot and mouth disease virus(FMDV):

作　　者：何永强[1] 杜青云[1] 盛祖恬[1] 

机构地区：[1]浙江省农业科学院病毒实验室,浙江杭州310021

出　　处：《浙江农业学报》2002年第3期135-138,共4页Acta Agriculturae Zhejiangensis

基　　金：浙江省自然科学基金资助项目 (3970 4 5 )

摘　　要：猪口蹄疫病毒毒株 (FMDV B ,FMDV D ,FMDV G ,FMDV S)经乳鼠和PK15传代细胞适应传代 ,通过差速离心和蔗糖密度梯度离心纯化抗原 ,免疫BALB/C小鼠 ,取脾细胞和SP2 /O在PEG4 0 0 0作用下融合 ,经间接免疫荧光技术检测 ,有限稀释法克隆 ,筛选得到 6株能特异分泌抗FMDV的单克隆抗体 (McAb)。其中McAb B4 3,McAb B6 2属IgM ;McAb D16 ,McAb G17属IgG1;McAb G5 2 ,McAb S2 5属IgG2b。在液氮中保存 6个月 ,单抗效价基本保持不变。McAb G17,McAb G5Strains B,D,G,S of foot and mouth disease virus(FMDV) were multiplied in murine and PK 15 cell.The myeloma cell line SP 2/O fused with the speen cell of BALB/C mice immunized with the FMDV antigen purified by differential centrifugation and sucrose density gradient centrifugation.By 3 times limiting dilution, six monoclonal antibodies(McAbs) specific for FMDV were screened by indirect immufluorecent technique (IIF). Among them, McAb B43, McAb B62 belonged to IgM,McAb D16, McAb G17 to IgG 1,McAb G52, McAb S25 to IgG 2b . Titer of McAbs was stable in liquid nitrogen for six months. McAb G17, McAb G52 had strong neutralization charactristics.

关 键 词：猪 口蹄疫病毒 单克隆抗体 生物学特性 

分 类 号：S858.28[农业科学—临床兽医学]