中华绒螯蟹血淋巴细胞的超微结构  

Ultrastructural study on hemocyte of Chinese mitten crab Eriocheir sinensis

作　　者：朱宁宁[1] 王文[1] 顾志峰[1] 李正荣[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097

出　　处：《中国水产科学》2002年第2期114-116,共3页Journal of Fishery Sciences of China

基　　金：江苏省水产三项工程项目 (Z2 0 0 10 1)

摘　　要：改进常规血液细胞电镜制样技术 ,直接取中华绒螯蟹 (Eriocheirsinensis)血淋巴液固定、脱水、包埋、电镜观察。结果显示 ,中华绒螯蟹血淋巴具有 3类细胞 ,即无颗粒细胞、小颗粒细胞和大颗粒细胞。其中 ,无颗粒细胞的核质比最大 ,异染色质高度浓缩、边缘化 ,几乎没有常染色质 ,胞质内线粒体数目相对较多 ,与高等动物的中幼红细胞结构很相似 ,该细胞数目在中华绒螯蟹血淋巴中最多 ,可能担负着血液的能量转换功能 ;小颗粒细胞核内常染色质较多 ,内质网、高尔基体发达 ,胞质中存在 0 .2～ 0 .4 μm的电子致密颗粒 ,而且胞质中有许多大小不一的空泡 ,细胞表面有许多突起似伪足 ,类似高等动物的单核细胞 ,可能具有吞噬和消化异物的功能 ;大颗粒细胞胞质中的颗粒较大 ,直径 1.4～ 2 .0 μm ,胞质中具空泡 ,细胞表面也常见伪足突起 。The samples of Eriocheir sinensis were collected from the farms in Jiangsu Province. Directly using the hemocyte of the crab to fix, dehydrate and embed,the ultra thin section was observed under electron microscope(Hitachi H-600). The results show that the hemocyte includes three types of cells,i.e. agranular hemocyte,small granular hemocyte and large granular hemocyte. The agranular hemocyte has the highest ratio of nucleus to cytoplasm (N/C) and the nucleus has no euchromatin but has heterochromatin condensing around the nuclear membrane,just like the rubricyte. There is a large number of mitochondria in the agranular hemocyte,which play the role of energy transform. There are a lot of euchromatin in the small granular hemocyte where the mitochondria and Golgi apparatus develop well and in the cytoplasm there exist electron dense granules of 0.2-0.4 μm in diameter and some vacuoles,and on the membrane of the cell often form the prominences and pseudopodiums. This type of cell has the similar characters to the monocyte,performing the function of phagorytosis. The large granular hemocyte has larger granules with diameter of 1.4-2.0 μm and with vacuoles in the cytoplasm and pseudopodiums on the cell surface.

关 键 词：淋巴细胞 中华绒螯蟹 血淋巴 超微结构 

分 类 号：Q959.223.5[生物学—动物学]