胆碱能受体激动剂和拮抗剂对体外CFU-GM增殖和分化的影响  

EFFECT OF CHOLINERGIC AGONIST AND ANTAGONIST ON PROLIFERATION AND DIFFERENTIATION OF CFU-GM IN VITRO

作　　者：邱立波[1] 徐有恒[1] 周衍椒[1] 唐恢玲 蒋德昭[1] 

机构地区：[1]湖南医科大学血液生理研究室

出　　处：《湖南医科大学学报》1989年第3期231-234,共4页Bulletin of Hunan Medical University

基　　金：国家自然科学基金

摘　　要：用体外琼脂培养法研究胆碱能受体激动剂和拮抗剂对粒、单造血祖细胞(CFU-GM)增殖和分化的影响。结果显示,10^(-10)-10^(-6)氨甲酰胆碱均能增加CFU-GM的产率,阿托品可以阻断这种效应;氨甲酰胆碱亦能增加CFU*GM的自杀率及培养基中粒细胞集落的比例。文中讨论了CFU-GM上胆碱能受体的功能特征。This study observed the effect of cholinergic agonist and antagonist on proliferation and differentiation of rganulocytemacrophage haemopoietic progenitor cells (CFU-GM) in vitro. The results showed dosage dependent enhancement of granulocytemacrophage colony growth after treatment of murine bone marrow cultrue with cholinergic agonist carbamylcholine (Cach) .Exposure of CFU-GM to Cach caused a marked increase in their sensitivity to the cytocidal action of cytosine arabinoside.On the other hand, the proportion of granulocytic colonies , which was identified by staining in situ, among the total CFU-GM colonies was also increased after administration Cach. Atropine but not d-Tubocurarine might abolish the above cell effects of Cach.These data indicate the presence of muscarine receptors on CFU-GM. Through activating muscarinic, receptors, Cach may trigger CFU-GM form Go into DNA synthesis phase, and induce differetiation of CFU-GM towards granulocytic cell line.

关 键 词：造血干细胞 胆碱能 氨甲酰胆碱 

分 类 号：R331.2[医药卫生—人体生理学]