转化生长因子β1与原因不明习惯性流产关系的初步研究  

Study on the relationship of transforming growth factor beta 1 and unexplained habitual abortion.

作　　者：邱丽华[1] 林其德[1] 李东至[1] 丁传伟[1] 洪 燕[1] 

机构地区：[1]上海第二医科大学附属仁济医院妇产科,上海200001

出　　处：《现代妇产科进展》2002年第3期203-205,共3页Progress in Obstetrics and Gynecology

基　　金：卫生部科研基金资助项目(98-2-301)

摘　　要：目的:探讨原因不明习惯性流产（UHA）患者主动免疫治疗前后外周血单个核细胞转化生长因子β1（TGF-β1）mRNA水平的变化。方法:用半定量逆转录-聚合酶链反应（RT-PCR）技术,检测正常非孕妇女30例和UHA妇女30例主动免疫治疗前和治疗后3周的外周血单个核细胞内TGF-β1　mRNA 表达水平的相对含量（％）。结果:UHA组外周血单个核细胞TGF-β1　mRNA的相对含量（123.2±62.7）％明显低于正常非孕组妇女（192.7±64.6）％（P<0.01）。主动免疫治疗后,UHA组TGF-β1mRNA的相对含量（172.2±74.9）％较治疗前明显升高（P<0.01）,且和正常非孕组差异无显著性。UHA组经主动免疫治疗后,16例妊娠成功,6例仍发生自然流产。妊娠成功者中TGF-β1　mRNA的相对含量（183.5±90.5）％较治疗前（113.6±68.6）％明显升高（P<0.05）,而妊娠失败者TGF-β1mRNA的相对含量与治疗前无显著差异。结论:亿卜p1表达水平降低可能与UHA的发生相关,主动免疫治疗可能通过上调TGF-β1的表达水平促使UHA患者获得妊娠成功。ve:To investigate the change of transforming growth factor beta 1 (TGF-β1) mRNA expression on peripheral blood mononuclear cells (PMBC) in unexplained habitual abor-tion (UHA) women before and after the active immunotherapy.Methods:The expression of TGF-β1 mRNA was measured by semi-quantitative reverse transcriptional-polymerase chain reaction (RT-PCR) on PMBC.The samples were taken from 30 normal non-pregnant (NNP) women and 30 UHA women before and three weeks after active immunotherapy. Results: TGF-β1 mRNA expression was lower significantly on PMBC in UHA women (123.2±62.7) % than that in NNP women (192.7±64.6)% (P < 0.01).Immunotherapy leads to a significant enhancement in TGF-β1 mRNA expres-sion (172.2±4.9)% vs (123.2±2.7)% (P < 0.01) .After immunotherapy, there was no sig-nificant difference in the expression of TGF-β1 mRNA between UHA women and NNP women. After immunotherapy, 16 women had successful pregnancy, and TGF-β1 mRNA expression was increased significantly [(183.5±90.5)% vs (113.6±68.6)% ] (P<0.05) in those women. However, 6 women still had spontaneous abortions, and no significant difference in TGF-β1 mRNA expression was found in those women. Conclusions: It suggests a potential mechanism of UHA which involves the reduced expression of TGF-β1, and the active immunotherapy can increase the expression of TGF-β1, thus favoring the maintenance of pregnancy.

关 键 词：习惯性流产 转化生长因子Β 主动免疫疗法 

分 类 号：R714.21[医药卫生—妇产科学]