hPER1-NLS:一种新型穿膜多肽的研究  

hPER1-NLS: study of a novel penetrating peptide

作　　者：郭勇[1] 程牛亮[1] 王晓霞[1] 刘志贞[1] 解军[1] 

机构地区：[1]山西医科大学生物化学与分子生物学教研室,太原030001

出　　处：《山西医科大学学报》2002年第3期197-198,共2页Journal of Shanxi Medical University

基　　金：山西省青年科研基金资助项目

摘　　要：目的　研究hPER1-NLS多肽的穿膜特性。方法　用固相法化学合成hPER1-NLS多肽及对照多肽 ,用免疫组化方法观察hPER1-NLS的穿膜能力及在细胞中的定位 ,并对其穿膜的特性做初步研究。结果　hPER1-NLS对细胞膜有穿透作用 ,主要定位在细胞核中。且在不同作用环境条件下 ,都具有穿膜能力。结论　hPER1-NLS也是一种MPP 。Objective To study property of penetrating membrane of hPER1-NLS. Method hPER1-NLS and other peptides were synthesized by solide-phase method and immunohistochemical technique was used to confirm its targeting of intracellular or intranuclear. Property of penetrating membrane of hPER1-NLS was studied in different conditions. Results hPER1-NLS possessed penetrating ability in different conditions and demonstrated intense focal staining in the nuclear plasma as well as in the nucleus. Conclusion It is implied that hPER1-NLS is also MPP, suggesting that hPER1-NLS is used in gene therapy and study of the mechanism of penetrating membrane.

关 键 词：hPER1-NLS 膜穿透肽 跨膜转运 免疫组织化学 研究 

分 类 号：Q735[生物学—分子生物学]