赛庚啶对胎鼠脑细胞内钙的影响  

Effects of cyproheptadine on intracellular free calcium in isolated embryonic rat brain cells

作　　者：张轩萍[1] 曹建民 牛拴成[3] 杨波[3] 汤允昭[1] 

机构地区：[1]山西医科大学药理学教研室,太原030001 [2]西山煤电集团公司杜儿坪矿医院放射科 [3]山西医科大学机能实验室

出　　处：《山西医科大学学报》2002年第3期209-211,共3页Journal of Shanxi Medical University

摘　　要：目的　研究赛庚啶对中枢神经系统的钙拮抗作用。方法　应用钙敏感的荧光指示剂Fura - 2 ,于双波长荧光分光光度计上测定胎鼠脑细胞内钙。结果　不同外钙条件下 (0 ,0 .0 1,0 .1,1.0 ,1.3mmol/L)脑细胞静息钙离子浓度分别为 41±6 ,5 8± 6 ,84± 3,10 4± 8,12 7± 11nmol/L。赛庚啶 0 .2mmol/L对静息钙离子浓度没有显著影响。但赛庚啶 (0 .0 5～ 0 4mmol/L)能浓度依赖性地抑制外钙 1.3mmol/L条件下高钾和L -谷氨酸引起的细胞内钙浓度增高。其IC50 值分别为 0 .135mmol/L(95 %的可信区间为 0 .0 83～ 0 .2 16mmol/L)和 0 .119mmol/L(95 %可信区间为 0 .0 70～ 0 .2 0 2mmol/L)。Objective To study the calcium antagonistic effects of cyproheptadine(CPH) in central nervous system(CNS). MethodsUsing calcium sensitive fluorescence indicator Fura-2 technique, the calcium concentration in isolated embryonic rat brain cells was determined. Results Resting [Ca 2+ ] i in brain cells under different extracellular calcium concentrations (0, 0.01, 0.1, 1.0, 1.3 mmol/L)were 41±6, 58±6, 84±3, 104±8 and 127±11 nmol/L, respectively(n=8). CPH 0.2 mmol/L had no significant effects on resting[Ca 2+ ] i. However, CPH (0.05～0.4 mmol/L) concentration-dependently inhibited the [Ca 2+ ] i elevation induced by high extracellular potassium or L-glutamate in the presence of extracellular Ca 2+ 1.3 mmol/L. The IC 50 values were 0.135 mmol/L(95% confidence interval 0.083～0.216 mmol/L) and 0.119 mmol/L(95% confidence interval 0.070～0.202 mmol/L) respectively. Conclusion It is suggested CPH has strong inhibitory effects on voltage-activated calcium channel and receptor-operated calcium channel in CNS.

关 键 词：赛庚啶 FURA-2 钙通道阻滞药 离子运转 大鼠 

分 类 号：R972[医药卫生—药品]