幽门螺杆菌热休克蛋白A与尿素酶B融合表达产物的初步纯化及其在小鼠体内免疫效应活性分析  

Purification and immunologic activity analysis of fusion expression protein of urease B and heat-shock protein A in Helicobacter pylori

作　　者：陶好霞[1] 刘纯杰[1] 李淑琴[1] 李责力 刘秀丽[1] 周围[1] 张兆山[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《军事医学科学院院刊》2002年第2期90-92,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：国家 8 63高技术基金资助课题 (0 0 1AA2 15 161) ;全军医学科研重点资助课题 (98Z0 71)

摘　　要：目的 :探讨幽门螺杆菌热休克蛋白A与尿素酶B融合蛋白的免疫生物学活性。方法 :采用亲和层析、离子交换和凝胶过滤对幽门螺杆菌热休克蛋白A与尿素酶B融合蛋白进行初步纯化 ,用粗纯化蛋白对小鼠进行免疫 ,然后分析其免疫活性。结果 :采用亲和层析或离子交换和凝胶过滤后 ,融合蛋白的纯度分别可达到 6 5 .6 %和90 .2 %。该融合蛋白可诱导小鼠产生抗幽门螺杆菌的胃肠黏液IgA和血清IgG抗体 ,并促使免疫小鼠脾脏T淋巴细胞CD4+ CD8+ 比值的增加。结论Objective:To analyze the immunobiological activity of fusion expression protein of urease B and heat_shock protein A in Helicobacter pylori . Methods:The fusion expression protein of urease B and heat_shock protein A in H.pylori was purified preliminarily with the methods of affinity chromatography, ion_exchange chromatography and gel filtration. After the purified protein immunized mice, their biological activities were analysed.Results:The purity of fusion protein was 65.6% and 90.2%,respectively after purified with affinity chromatography or ion_exchange chromatography and gel filtration methods. They can induce mice to produce IgA of gastrointestinal secretions and serum anti _ H.pylori IgG antibodies and increased mice spleen T lymphocytes CD 4 +/CD 8 + ratio. Conclusions:The fusion expression protein of urease B and heat_shock protein A in H.pylori can induce mice to produce an active immunity reply reaction in future immunologic prophylaxis.

关 键 词：幽门螺杆菌 热休克蛋白A 尿素酶B 融合表达产物 纯化 小鼠 免疫效应 

分 类 号：R392[医药卫生—免疫学]