端粒酶催化亚基基因表达激活人胚肺成纤维细胞端粒酶活性  

Telomerase activation in human fibroblasts by expressing the catalytic component of human telomerase

作　　者：郑晓飞[1] 朱捷[1] 杨义军[2] 江红[1] 吕星[1] 孙志贤[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]沈阳军区军事医学研究所,沈阳110031

出　　处：《军事医学科学院院刊》2002年第2期96-98,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：国家重点基础研究规划资助项目 (G2 0 0 0 0 5 70 0 1) ;国家自然科学基金资助项目 (3 0 0 70 85 9)

摘　　要：目的 :通过端粒酶催化亚基基因转入人胚肺成纤维细胞 ,激活人胚肺成纤维细胞端粒酶活性 ,探讨其在延缓细胞衰老中的作用。方法 :用PCI_hTRT质粒转染人胚肺成纤维细胞 ,G4 18筛选获得稳定表达端粒酶活性的人胚肺成纤维细胞 ,采用RT_PCR和TRAP法分别测定端粒酶催化亚基mRNA和端粒酶活性表达情况。结果 :获得了表达端粒酶活性的人胚肺成纤维细胞 ,其寿限长于正常人胚肺成纤维细胞。结论 :端粒酶催化亚基异位表达可以激活人胚肺成纤维细胞的端粒酶活性 。Objective:To investigate the role of telomerase activation in aging by maintaining cultures of hTRT_transduced human fetal lung fibroblasts. Methods:Following delivery of the hTRT gene to human fetal lung fibroblasts using a PCI_hTRT vector, cell clones that expressed functional telomerase activity were screened by G418. Telomerase activity, hTR RNA and hTRT mRNA in normal fibroblasts and hTRT_transduced human fetal lung fibroblasts were investigated.Results: Human fetal lung fibroblasts clones with positive telomerase were obtained, and the cellular life span of hTRT_transduced human fetal lung fibroblasts was extended.Conclusions:Ectopic expression of telomerase (hTRT) in human fetal lung fibroblasts has been shown to restore telomerase activity and to extend cellular life span.

关 键 词：端粒酶催化亚基 人胚肺 端粒酶 成纤维细胞 细胞衰老 基因表达 

分 类 号：Q255[生物学—细胞生物学]