^(188)Re直接法标记生长抑素类似物RC-160及其体内分布研究  

Direct labeling of RC-160 with ^(188)Re and the biodistribution of ^(188)Re-RC-160 in mice

作　　者：汪静[1] 王连刚[2] 邓敬兰[1] 李富军[1] 

机构地区：[1]第四军医大学西京医院核医学科,西安710032 [2]第四军医大学西京医院实验外科,西安710032

出　　处：《中华核医学杂志》2002年第3期177-178,共2页Chinese Journal of Nuclear Medicine

基　　金：国家自然科学基金资助项目 ( 39870 2 18) ;全军医药卫生科研基金资助项目 ( 0 1MB12 0 )

摘　　要：目的　探讨188Re直接法标记生长抑素类似物RC 16 0的方法及观察其在小鼠体内的生物学分布。方法　酒石酸亚锡直接还原法进行RC 16 0的188Re标记 ,标记完成后加入抗坏血酸以防标记产物的再氧化。硅胶纸层析测定放化纯 ,并行小鼠体内分布实验。结果　188Re RC 16 0放化纯为 96 .2 % ,游离188Re为 0 .8% ,放射性胶体为 3.0 %。加抗坏血酸组在标记后 2 4h放化纯为 85 % ,对照组为 5 0 %。注射后 0 .5～ 1.0h血液中放射性下降了 87.2 % ,肾脏无明显浓集 ,标记物 2 4h内由消化系统排出体外。结论　188Re直接法标记RC 16 0放化纯高 ,方法简便 ,抗坏血酸的加入增加了标记物的稳定性。188Re RC 16 0在小鼠体内血液清除较快 ,经消化系统排除。Objective To investigate labeling method of RC-160 with 188 Re and the biodistribution of 188 Re-RC-160 in mice. Methods RC-160 was labeled with 188 Re through stannous tartrate reduction at room temperature under nitrogen gas condition. Ascorbic acid was added to inhibit re-oxidation of 188 Re-RC-160. The radiochemical purity was tested with silicon paper chromatography. Its biodistribution in normal mice was analyzed. Results The radiochemical purity was 96.2%. Free 188 Re was less than 0.8%. Radioactive colloid was 3%. The radiochemical purity at 24 h after labeling was 85% in ascorbic acid group while 50% in control group. At 0.5～1.0 h after injection, radioactivity in blood decreased by 87.2%. No obvious accumulation of 188 Re-RC-160 in the kidney was observed. A great amount of radioactivity was detected in gastrointestinal system within 12～24 h. Conclusions The method is simple and easy to perform. The radiochemical purity is quite high. The stability of 188 Re-RC-160 is improved by adding ascorbic acid. Blood clearance of 188 Re-RC-160 in normal mice is quite fast. 188 Re-RC-160 is excreted through gastrointestinal tract.

关 键 词：^188RE直接法 标记 生长抑素类似物 RC-160 体内 分布 研究 放射性同位素 同位素标记 药代动力学 

分 类 号：R817[医药卫生—影像医学与核医学]