SP法检测死胎心脏组织中HBcAg表达的初步探讨  

The primary study on the expression of HBcAg in the cardiac tissues of the dead fetus

作　　者：陈宏[1] 赵伟[1] 刘伟[1] 罗婵[1] 

机构地区：[1]南京东南大学医学院附属南京第二医院病理科,南京210003

出　　处：《胃肠病学和肝病学杂志》2002年第2期152-153,共2页Chinese Journal of Gastroenterology and Hepatology

基　　金：铁道部 2 0 0 0年自然科技资金资助 (J2 0 0 0Z0 95)

摘　　要：目的　观察乙型肝炎病毒通过产妇传播到胎儿心脏组织的表达情况。方法　采集 40例乙型肝炎产妇产下的死胎 ,常规尸检 ,取心脏组织 ,SP法检测HBcAg ;回访婴母产前静脉血HBV的检测结果 ,取百分率行u检验。结果　HBcAg阳性颗粒在死胎的心内膜上、心脏血管中点、灶状分布 ,心肌细胞核不着色。HBV呈大三阳的婴母较HBV呈单项阳性、小三阳的婴母分娩的死胎心脏组织中HBcAg阳性率明显升高 ,差异显著 (P <0 0 0 5 ) ;HBV呈单项阳性、小三阳的婴母分娩的死胎心脏组织中HBcAg阳性率比较 ,差异不显著 (P >0 0 5 )。结论　婴母静脉血HBV大三阳是乙型肝炎病毒通过母婴垂直传播在胎儿心脏组织中表达的高危因素。Aim To observe the expression of HBV in the fetus cardiac tissue by maternal fetal transmission. Methods Forty heart tissues of dead fetus that the puerperant with hepatitis virus B deliveried were made autopsy and detected HBcAg by immunocytochemistry(SP). HBV in the blood of parturients was detected before delivery.Results There was HBcAg in the cardiac tissues and HBcAg mainly distributed on the endocardium and cardiovascular vessel,not in the cardiac muscle cells nuclei.The positive cells were single or in clusters.There was statistically significant ( P <0 005) in the positive rate of the HBcAg expression in the cardiac tissues of the dead fetus that was deliveried by the parturients with hepatitis virus B between①HBsAg?②(HBsAg+HBcAb+HBcAb) and ③(HBsAg+HBeAg+HBcAb).The comparison between ①and ②was not statistically significant ( P >0 05).Conclusion It is a high risk factor for the cardiac tissue of the dead fetus infected by HBcAg by maternal fetal transmission that HBV M (HBcAg+HBeAg+HBcAb) is positive expression in the blood of the parturients before delivery.

关 键 词：SP法 检测 表达 死胎 心脏组织 HBCAG 母婴传播 

分 类 号：R512.62[医药卫生—内科学]