大鼠梗阻性黄疸形成中血及肝组织内皮素的水平及意义  

Level changes and significance of endothelin in plasma and liver tissue during formation of obstructive jaundice in rats

作　　者：李端阳[1] 吴金生[2] 马庆久[2] 高德明[2] 褚延魁[2] 

机构地区：[1]武警江苏总队南京医院普通外科,南京市210028 [2]第四军医大学唐都医院普通外科,西安市710038

出　　处：《中华肝胆外科杂志》2002年第6期358-361,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的　了解梗阻性黄疸 (obstructivejaundice ,OJ)形成过程中内皮素 (endothelin ,ET)在血浆及肝组织中的水平及其在OJ肝损伤中的作用。方法　以大鼠胆总管结扎 (bileductligation ,BDL)为模型 ,采用放射免疫法 (radioimmunassay ,RIA)测定了BDL后 3、6、9、1 2、1 5d时血浆及肝组织中ET的含量 ,同时观测血清谷丙氨酸转氨酶 (ALT)含量变化 ,并与肝组织病理改变进行对照分析。结果　BDL后血清总胆红素 (totalbilirubin ,TBi)迅速升高 ,明显高于NC组和SO组 (P <0 0 1 ) ;BDL组血浆及肝组织中ET水平明显高于对照组 (P <0 0 1 ) ,BDL各时相组差异有显著性意义 (F =1 8 41 ,P <0 0 1和F =40 61 ,P <0 0 1 ) ,随梗阻时间延长呈进行性含量升高 ,1 5dET值最高 ,分别为 (2 94 98± 35 95) pg/ml和 (2 0 1 86± 2 4 45) pg/ 1 0 0mg ;二者与血清ALT均呈正相关 (r =0 8441 ,P <0 0 1和r=0 930 3 ,P <0 0 1 ) ,病理切片见肝细胞从肿胀、空泡变性到灶状片状坏死 ,损伤也呈进行性加重 ,与ET含量变化一致 ;病理切片还显示肝内有大量的小胆管及毛细胆管的增生 ,且随着胆管梗阻时间的延长 ,增生越来越明显。结论　实验结果提示在OJ形成中血及肝组织中ET含量上升 ,并随梗阻时间延长而加重 ;ET可能是参与OJ肝脏?Objective To investigate changes in endothelin level in plasma and liver tissue and evaluate the role of endothelin in liver damage during formation of obstructive jaundice in rats. Methods The concentration of endothelin in plasma and liver tissue was determined with radioimmunoassay on the 3rd, 6th, 9th, 12th and 15th day, respectively, after bile duct ligation (BDL). Meanwhile, the histopathological changes of liver tissue were observed. Results Serum total bilirubin was elevated rapidly after BDL. In addition, it was markedly higher than those in the control and sham-operated groups (P<0 01). The concentration of endothelin was remarkably increased after BDL as compared with the control and sham-operated groups (P<0 01) and was elevated with time prolongation. The peak values appeared on the 15th day after BDL (294 98±35 95 pg/ml and 201 86±24 45 pg/100 mg, respectively). Endothelin levels of plasma and liver tissue were well correlated to serum level of ALT (r=0 8441, P<0 01 and r=0 9303, P<0 01 respectively). Histological examination showed that liver cells were damaged and the damage was progressively consistent with concentration of endothelin with BDL prolongation. There was significant cholangiohyperplasia and its changing rules were quite similar to those of endothelin levels. Conclusions Endothelin levels in plasma and liver tissue significantly increased in BDL and may be an important factor inducing liver damage in obstructive jaundice. Furthermore, it is strongly related to cholaniohyperplasia.

关 键 词：大鼠 梗阻性黄疸 血 肝组织 内皮素 胆汁淤积 谷丙氨酸转氨酶 

分 类 号：R657.43[医药卫生—外科学]