反相高效液相色谱法测定梅花点舌丸中酯蟾毒配基、华蟾毒配基的含量  

Determination of Resibufogenin and Cinobufagin in Meihuadianshe Pills by RP-HPLC

作　　者：李亚芳[1] 胡晓炜[2] 

机构地区：[1]杭州市第一人民医院,杭州市310006 [2]杭州市药品检验所,杭州市310014

出　　处：《中国药房》2002年第6期362-363,共2页China Pharmacy

摘　　要：目的 :建立测定梅花点舌丸中酯蟾毒配基、华蟾毒配基的反相高效液相色谱法。方法 :采用KromasilC18 柱 ,流动相为乙腈∶0 5 %KH2PO4 溶液 (50∶50) ,检测波长为296nm。结果 :该方法线形关系良好 ,酯蟾毒配基的平均加样回收率为97 40 % ,RSD为2 2 % ;华蟾毒配基的平均加样回收率为97 78 % ,RSD为2 6 %。结论 :方法简便可靠 ,分离度较好 ,可用于梅花点舌丸的质量控制。OBJECTIVE:To establish a RP-HPLC method for determination of the contents of resibufogenin and cinobufagin in Meihuadianshe pills METHODS:The condition of HPLC was Kromasil C18 column,acetonitrile-0 5%KH2PO4(50∶50) as a mobile phase and detecting wavelength 296nm RESULTS:This method showed good linearity The average recoveries of resibufogenin and cinobufagin were 97 40% with a RSD of 2 2% and 97 78% with a RSD of 2 6% respectively CONCLUSION:The method is simple,reliable and satisfactory in resolution It can be used in quality control of Meihuadianshe pills

关 键 词：梅花点舌丸 酯蟾毒配基 华蟾毒配基 反相高效液相色谱法 含量 测定 中药 

分 类 号：R284.1[医药卫生—中药学]