非复制重组痘苗病毒中白细胞介素6的表达及其对重组病毒免疫效果的影响  

Expression of IL-6 in recombinant non-replicating vaccinia virus and studying its effects on immunogenicity

作　　者：郭斐[1] 陆柔剑[1] 孙朝晖[1] 马海伦[1] 李军[1] 阮力[1] 张颖妹[2] 马大龙[2] 

机构地区：[1]中国预防医学科学院病毒学研究所遗传免疫室,北京100052 [2]北京医科大学免疫系

出　　处：《中华实验和临床病毒学杂志》2002年第2期136-141,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：国家"八六三"高技术研究发展计划 (86 310 2 0 7 0 20 1);国家自然科学基金( 3952 50 0 1);国家自然科学基金(30 0 70 711)

摘　　要：目的　研究白细胞介素 6(IL 6)在非复制型痘苗病毒中的表达及其对重组痘苗病毒免疫效果的影响。方法　利用非复制型痘苗病毒表达载体pNEOCK1 1 β75IL6和重组病毒RVJ1 2 3 ,通过两步重组构建能同时表达IL 6和乙型肝炎病毒 (HBV)HBsAg的非复制型重组痘苗病毒RVJ1 2 3ΔCK1 1 β75IL6。免疫动物并观察其免疫效果。 结果　Southernblot证实痘苗病毒C、K片段间基因缺失的同时伴有IL 6基因的插入 ,IL 6和HBsAg可同时表达。鼻腔吸入分别免疫BALB c小鼠和新西兰白兔 ,ELISPOT实验证实免疫后两周小鼠肺淋巴细胞的抗 HBsAgIgA、IgG抗体分泌细胞 (ASC)数比对照组 (RVJ1 2 3ΔCK1 1 β75)显著增加 ,并可在小鼠血液、肺浸出液以及新西兰白兔血液、肺浸出液、其他分泌液样品中检测到抗 HBsAg的特异性的IgA、IgG抗体。与对照组相比 ,IgA、IgG抗体阳转率及抗体滴度提高。结论　非复制型痘苗病毒载体中表达的IL 6可增强其诱导的免疫反应 ,为细胞因子ILObjective To investigate expression of IL 6 in non replicating vaccinia virus and its immune effects on recombinant virus.Methods The recombinant non replicating vaccinia virus RVJ123ΔCK11β75IL6 was constructed with non replicating vaccinia virus vector pNEOCK11β75IL6 and replicating vaccinia virus RVJ123.In animal model,immunization with the recombinant virus was carried out and its immune response was studied. Results The recombinant virus could express IL 6 and HBsAg simultaneously.Southern blot analysis demonstratecd that the genes between vaccinia virus HindⅢ C and K fragments were deleted and IL 6 gene was integrated stably.Given intranasal inocula of the virus to immunize BALB/c mouse and New Zealand Rabbit,the elevated anti HBsAg IgA and IgG antibody secreting cells in mouse lung lymphoid to vectors expressing IL 6 was at about two fold higher level than those elicited by contorl virus at day 14 after immunization.Authors also could detect elevated anti HBsAg IgA and IgG antibody conversion in mouse serum and lung fluid,rabbits serum,lung fluid,saliva,vagina and nasal washing samples.Conclusion IL 6 expressed by non replicating recombinant vaccinia virus could enhance the induced immune effects,it could serve as the effective adjuvant for recombinant vector vaccine.

关 键 词：白细胞介素6 病毒痘苗 免疫佐剂 

分 类 号：R392[医药卫生—免疫学]