Sybr green1实时定量PCR检测乙型肝炎病毒DNA的探索  

Detection of the HBV DNA with Sybr green 1 real-time polymerase chain reaction

作　　者：王艳[1] 徐小元[1] 公维波[1] 王勤环[1] 何为[2] 刘志红[2] 

机构地区：[1]北京大学第一医院感染疾病科,北京100034 [2]军事医学科学院放射医学研究所

出　　处：《中华实验和临床病毒学杂志》2002年第2期160-161,共2页Chinese Journal of Experimental and Clinical Virology

基　　金：国家教委博士点基金( 992 4 )

摘　　要：目的　寻找一种能广泛用于临床快速、简便、灵敏度和特异性较好、价格低廉的乙型肝炎病毒 (HBV)DNA定量诊断方法。方法　以Sybrgreen 1作为荧光指示剂 ,做样品血清DNA的实时定量聚合酶链反应 (PCR) ,并结合溶解曲线结果 ,与Taqman荧光定量方法结果进行了对比。结果　Taqman法较好地检出体内HBVDNA载量。Sybr法测得样品的病毒拷贝数 5 3× 1 0 4copies ml与Taq man法所得数值 9 6× 1 0 4copieslml相近 ,但检出率偏低。结论　Sybrgreen 1荧光实时定量方法简便、便宜 。Objective To search a novel sensitive,specific and lower cost method applicable for quantitative analysis of the hepatitis B virus DNA extensively.Methods Quantitative analysis of the DNA from 100 sera by real time PCR with Sybr green 1. The results of Sybr's assay were compared with the results obtained with Taqman's fluorescent quantilalive assay.Results Taqman real time PCR could help evaluate the level of virus reliably. The results of Sybr's assay were in agreement with the Taqman's assay,but detection rate was lower.Conclusion Sybr green 1 real time PCR appeared to be convenient and cheap,but detection rate was lower.

关 键 词：乙型肝炎 病毒DNA 聚合酶链反应 荧光 溶解曲线 

分 类 号：R446.5[医药卫生—诊断学]