乙型肝炎病毒C基因启动子变异株克隆的构建及应用  

Construction of HBV Basic Core Promoter Mutants

作　　者：陆建荣[1] 

机构地区：[1]江苏省南通市第三人民医院病毒性肝炎研究室,226006

出　　处：《齐齐哈尔医学院学报》2002年第3期246-247,共2页Journal of Qiqihar Medical University

摘　　要：目的　构建乙型肝炎病毒 (HBV)C基因启动子 (BCP)变异株克隆。方法　采用PCR产物克隆法。结果　构建成功 pBCM质粒 ,经克隆鉴定及错配PCR -RFLP证实为目的克隆。 结论　质粒pBCM可作为错配PCR -RFLP检测HBVBCP基因变异株检测的阳性对照。Objective To construct hepatitis B virus(HBV) basic core promoter(BCP) mutants. Methods Mismatched primer polymerase chain reaction products were digested and cloned into pUC18.Results After clone identification and mismatched primer polymerase chain reaction-restriction fragment length polymorphism(mpPCR-RFLP) analysis, it was proved to be the HBV BCP mutant clone. Conclusions pBCM can be used as the standard strain to control the detection of HBV BCP mutants.

关 键 词：乙型肝炎病毒 C基因启动子 变异 克隆 

分 类 号：R373.21[医药卫生—病原生物学]