苯接触人群中GSTμ基因的多态性分析  

ANALYSIS ON GLUTATHIONE S-TRANSFERASEμ POLYMORPHISM IN THE POPULATION EXPOSED TO BENZENE

作　　者：王文静[1] 项翠琴[1] 陈纪刚[1] 张云英[1] 

机构地区：[1]上海市疾病预防控制中心,上海200336

出　　处：《职业卫生与应急救援》2002年第2期57-59,共3页Occupational Health and Emergency Rescue

摘　　要：探讨苯中毒患者GSTμ 基因缺失频度 ,为进一步研究GSTμ 基因缺失与苯白血病之间的关系寻找线索。方法采用聚合酶链反应(PolymeraseChainReaction,PCR)技术检测GSTμ 基因型。结果对照组、苯接触组、轻度苯中毒组、中度苯中毒组、重度苯中毒组GSTμ 基因缺失频率分别为46.88 %、51.79 %、53.75 %、56.00 %、69.84 %。进行2×K表的 χ2 检验结果显示:χ2=9.6555 ,P<0.05 ,说明苯接触者中毒的程度与GSTμ 基因缺失有关。结论GSTμ 基因缺失可能是苯中毒的危险性因素之一。Objective To explore the difference of GSTμ gene deficiency among the groups of workers exposed to benzene. Methods Polymerase chain reaction (PCR) method was used to investigate the rates of GSTμ gene deletion in workers exposed to benzene. Results The rate of GSTμ gene deletion in patients with severe benzene intoxication, moderate benzene intoxication, mild intoxication and benzene exposure, was 69.84%, 56.00%, 53.75% and 51.79%, which was significantly higher than that of the controls, 46.88%, P<0.05. The rates of GSTM1 gene deletion in the groups were increased with the severity of poisoning. An analysis of correlation was conducted that its coefficient of correlation was statistically significant difference (r=1.92, P<0.05). GSTμ gene deletion may be an important host hereditary marker for susceptibility to leukemia exposed to benzene. The rate of GSTμ gene deletion in leukemia patients exposed to benzene was 73.53%, which was significantly higher than of the controls (44.44%), P<0.01. Conclusion GSTμ gene deletion may be an important host hereditary marker for susceptibility to intoxication exposed to benzene.

关 键 词：谷胱甘肽巯基转移酶 GSTμ基因缺失 苯中毒 PCR技术 苯白血病 

分 类 号：R135.12[医药卫生—劳动卫生]