CpG序列和猪白细胞介素6基因转染表达对猪囊尾蚴基因疫苗免疫影响的研究  

The Effect of Transgenic Expression of Porcine Interleukin-6 Gene and CpG Sequences on Immune Responses of Mice Inoculated with the Gene Vaccine of Taenia Solium of Pig

作　　者：李江凌[1] 高荣[1] 武梅[1] 孟明杰[1] 龙章富[1] 唐漫书[1] 沈翼[1] 谢鸿观[1] 王丽焕[1] 巫雪艳[1] 殷雪[1] 刘世贵[1] 

机构地区：[1]四川大学草原生物防治工程国家专业实验室,成都610064

出　　处：《高技术通讯》2002年第6期26-30,共5页Chinese High Technology Letters

基　　金：国家教育部 2 0 0 0年重点资助项目

摘　　要：以猪囊尾蚴VTS76抗原基因和质粒VPIL 6、pUCl8 CpG免疫接种小鼠 ,检测了小鼠的体液及细胞免疫反应。结果发现 ,VPIL 6或pUC CpG与VTS76共同免疫小鼠的特异性抗体滴度、IgG含量、淋巴细胞白细胞介素 2诱生活性、脾细胞增殖反应和血液免疫细胞数量显著高于VTS76免疫组和对照组。证明VPIL 6和CpG序列能显著增强动物对DNA疫苗的细胞和体液免疫反应 。Porcine interleukin 6 gene and CpG sequences were firstly used as immunoadjuvants to enhance the immune responses of mice to VTS76, which is an antigenic gene of Taenia solium of pig cloned into VR1020 plasmid. The numbers of white blood cells, lymphocytes, monocytes and neutrophiles, the amount of IgG, the bioactivity of IL 2 and proliferation of lymphocytes have been detected to identify the immune response in peripheral blood and spleen of BALb/c mice. The results were found that the IgG contents and titre of specific antibodies to VTS76 antigen, the proliferation of lymphocytes, induced bioactivity of interleukin 2 and the numbers of immune cells were significantly increased in the mice immunized with porcine IL 6 and CpG motifs. All these suggested that porcine IL 6 gene and CpG motifs could be employed as effective immunoadjuvant to raise the humoral and cellular immune responses of animals to DNA vaccine.

关 键 词：疫苗 免疫 免疫应答 猪白细胞介素-6基因 CPG序列 猪囊尾蚴 抗原基因 免疫增强剂 基因表达 

分 类 号：S852.5[农业科学—基础兽医学]