大鼠5 Gy全身照射对伤口成纤维细胞功能的抑制效应及W_(11)-a_(12)的促愈作用  

Inhibitory effect of 5 Gy total-body irradiation and promoting role of W_(11)-a_(12) on wound fibroblast function in rats

作　　者：舒崇湘[1] 程天民[1] 李树楠[2] 冉新泽[1] 

机构地区：[1]第三军医大学预防医学系全军复合伤研究所 [2]云南大理医学院药理学教研室

出　　处：《中华放射医学与防护杂志》2002年第3期170-172,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家重点基础研究发展规划"973"专项经费资助项目 (G19990 5 42 0 5 ) ;重庆市科委院士基金资助项目 ( 98 5 0 81)

摘　　要：目的　探讨 5Gy全身照射对伤口成纤维细胞 (fibroblasts,FBs)功能的抑制效应及W1 1 a1 2 的促愈作用。方法　采用60 Coγ射线 5Gy全身照射大鼠复合背部植入聚乙烯醇海绵的皮肤伤口模型 ,观察伤后伤口FBs的纤维连接蛋白 (FN)、转化生长因子 β1 (TGFβ1 )、碱性成纤维细胞生长因子(bFGF)免疫细胞化学染色反应的变化及W1 1 a1 2 对其的影响。结果　 5Gy全身照射复合皮肤创伤后第 3、5天伤口内FN、TGFβ1 、bFGF免疫细胞化学染色反应阳性的FBs数量均显著减少 ,但减少的程度有所不同 ,由重到轻依次为bFGF、FN、TGFβ1 。而W1 1 a1 2 能显著增加未照射组和照射组伤口中FN、TGFβ1 、bFGF免疫细胞化学染色反应阳性的FBs数量。结论　全身放射损伤对伤口中FBs的合成与分泌功能具有抑制作用 ,增强FBs合成和分泌功能是W1 1 a1 2 促愈作用的一个途径。Objective\ To investigate the inhibitory effects of 5 Gy whole body irradiation on wound fibroblast function and the promoting role of W\-\{11\} a\-\{12\} on these cells. Methods\ Two full thickness skin incision wounds,in which polyvinyl alcohol sponges were planted,were inflicted on both sides of the back of rats after they had received 5 Gy gamma ray whole body irradiation.The percentages of immunocytochemistry positive fibroblasts for fibronectin (FN),transforming growth factor β\-1(TGFβ\-1) and basic fibroblasts growth factor (bFGF) in the wound were observed. Results\ The percentages of immunocytochemistry positive fibroblasts for FN,TGFβ\-1 and bFGF in wound were all significantly reduced on day 3 and 5 after 5 Gy whole body irradiation,but the degree of reduction varied in severity (bFGF>FN>TGFβ\-1).W\-\{11\} a\-\{12\} could increase the percentages of immunopositive cells for FN,TGFβ\-1 and bFGF both in simple wound and in wound impaired by irradiation. Conclusion\ Whole body irradiation injury could cause significant inhibitory effect on wound fibroblast function of synthesizing and secreting FN,TGFβ\-1 and bFGF.W\-\{11\} a\-\{12\} could promote irradiation impaired wound healing by means of enhancing these functions of wound fibroblasts.

关 键 词：5Gy全身照射 伤口 成纤维细胞功能 抑制效应 W11-A12 放射损伤 创伤愈合 纤维粘连蛋白 生长因子 

分 类 号：R818[医药卫生—放射医学]