5.21Gy软X射线局部照射伤口后组织细胞周期时相变化的研究  

Phase-changes in cell cycle of wound tissue irradiated with 5.21 Gy soft X-rays

作　　者：刘建忠[1] 周元国[1] 程天民[2] 周萍[1] 刘霞[1] 李平[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所分子生物学中心,重庆400042 [2]第三军医大学预防医学系全军复合伤研究所,重庆400042

出　　处：《中华放射医学与防护杂志》2002年第3期180-183,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家重点基础研究发展规划"973"专项经费资助项目 (G19990 5 42 0 5 )

摘　　要：目的　研究软X射线局部照射后大鼠伤口组织细胞周期的时相变化。方法　PI染色 ,流式细胞术 (FCM)检测细胞周期 ;BrdU掺入后采用SABC染色检测细胞增殖。结果　在伤后 3～9d ,对照侧G0 G1 期细胞逐渐减少 ,S期细胞增加 ,9d时达到峰值 ;G2 M期细胞增加 ,15d时达到峰值。照射侧G0 G1 期细胞增加 ,S期和G2 M期细胞减少。在伤后 12～ 2 2d ,照射侧S期细胞逐渐增加 ,2 2d时达到峰值 ,大量的细胞停留在S期。在整个愈合过程中 ,G2 M期细胞百分数均低于对照侧。BrdU掺入SABC染色阳性细胞主要为成纤维母细胞、血管内皮细胞以及平滑肌细胞。结论　5 2 1Gy软X射线局部照射后 ,细胞发生G1 期阻滞和S期延长 ,G2 M转换障碍 ,细胞分裂增殖受抑 ,创伤愈合延迟与细胞周期的紊乱有关 ,G1 期阻滞和S期延长越严重 ,愈合延长时间越长。Objective\ To study the phase changes in cell cycle of wound tissue which was locally irradiated with 5\^21 Gy soft X rays. Methods\ Flow cytometry and PI staining were used to analyze cell cycle.Cell proliferation was determined with BrdU labeling. Results\ During 3 9 days after irradiation,the percentage of the G\-0/G\-1 phase cells in wound of the control side decreased while the percentage of S phase cells increased and reached the highest value on day 9.The percentage of G\-2/M phase cells also increased,and reached its peak on day 15.The percentage of G\-0/G\-1 phase cell increased in wound of the irradiation side and was higher than that of the control wound,meanwhile the percentages of S and G\-2/M cells were significantly lower than those of the control wound.In the period of 12 22 days after wounding,the percentage of S phase cells increased and reached its peak value on the 22nd day.When most of cells were in S phase and arrested dramatically.Through the whole healing process,the percentage of G\-2/M in wound of the irradiation side was lower than that of the non irradiated wound.The BrdU positive cells were fibroblasts,endothelial cells and smooth muscle cells. Conclusion\ These results suggest that G\-1 block,S phase arrest,and switch of G\-2/M with suppression of mitotic activity of these cells are induced by local 5\^21 Gy soft X ray irradiation.Therefore,wound healing delay is induced partly by cell cycle arrest.

关 键 词：软X射线局部照射 细胞周期 时相变化 创伤愈合 

分 类 号：R14[医药卫生—公共卫生与预防医学]