SDF-1的表达与造血细胞回巢关系的实验研究  

Experinmental study on relationship between expression of SDF-1 and homing of hematopoietic cells

作　　者：孙苏平[1] 蔡建明[1] 项莺松[1] 赵芳[1] 黄定德[1] 高建国[1] 杨如俊[1] 

机构地区：[1]第二军医大学放射医学教研室,上海200433

出　　处：《中华放射医学与防护杂志》2002年第3期201-203,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 ( 39770 32 9;396 0 0 77)

摘　　要：目的　探讨趋化蛋白SDF 1在造血细胞回巢中的作用。方法　采用异基因小鼠骨髓细胞移植动物模型和免疫组化双染色方法 ,观察趋化蛋白SDF 1的表达与回巢细胞的关系。结果　骨髓内SDF 1主要表达在骨内膜旁、微血管内皮、骨细胞内和供体阳性细胞周围 ,与非照射组相比 ,照射预处理组骨髓中的SDF 1在骨细胞中、骨内膜旁有明显高表达 ,提示放射预处理可能促使髓内各种基质细胞分泌SDF 1,SDF 1与回巢细胞的关系密切。结论　照射预处理可能是增加骨髓基质细胞分泌SDF 1的激发因素之一 ,SDF 1在造血细胞回巢中有重要作用。Objective\ To investigate the role of chemoattractant SDF 1 in of homing hematopoietic cells. Methods\ A mouse allogeneic bone marrow transplantation model and double staining of immunohistochemistry were used in this study.Relationship between expression of chemoattractant SDF 1 and of homing hematopoietic cells was observed. Results\ In bone marrow,SDF\|1 was mainly expressed at sites near endosteum,in microvessel endothelium,osteocyte and around donor cells.Contrast to non\|irradiation group,SDF\|1 was significantly expressed by osteocytes,and at sites near endosteum in the irradiation group, suggesting that pretreatment with irradiation might enhance secretion of SDF\|1 by bone marrow stromal cells and SDF\|1 could have a close relation with homing cells. Conclusion\ Pretreatment with irradiation might be one of the stimulating factors significantly increasing the level of SDF\|1 produced by bone marrow stromal cells and the chemoattractant SDF\|1 could play an important role in homing of hematopoietic cells.;

关 键 词：SDF-1 造血细胞回巢 实验研究 异基因小鼠 骨髓移植 基因表达 

分 类 号：R457.7[医药卫生—治疗学]