锰对细胞免疫功能的影响  

Effects of Manganese on Cell-mediated Immune Function

作　　者：刘凤娟[1] 黄贤仪[1] 张月明[1] 姚海英[1] 

机构地区：[1]新疆医科大学公共卫生学院,新疆乌鲁木齐830054

出　　处：《环境与职业医学》2002年第3期139-142,共4页Journal of Environmental and Occupational Medicine

摘　　要：[目的 ]探讨锰对细胞免疫功能的影响。 [方法 ]利用体外培养的淋巴细胞 ,采用正交试验设计 ,观察不同染锰浓度和时间对淋巴细胞功能的影响。用溴化四氮唑盐检测法 (MTT法 )测定T淋巴细胞增殖功能和自然杀伤细胞 (NK细胞 )杀瘤细胞活性 ;用酶联免疫吸附试验 (ELISA)测定培养液中的白细胞介素 2 (IL 2 )含量。 [结果 ]随着培养液中的锰离子浓度由 1× 10 -6mol/L增大到 1× 10 -3 mol/L ,染锰时间由 2h延长到 4h ,T淋巴细胞增殖功能、NK细胞杀瘤细胞活性和IL 2含量均显著下降。 [结论The effects of manganese on cell-mediated immune function were studied. Lymphocytes were exposed to different concentration and duration of manganese in vitro using orthogonal design. Lymphocyte proliferation and natural killer cell activity were assessed with MTT assay,and the concentration of IL-2 was determined by ELISA. Lymphocyte proliferation,natural killer cell activity and the concentration of IL-2 significantly decreased when concentration of manganese ion increased from 1×10 -6 mol/L to 1×10 -3 mol/L,and treatment duration was prolonged from 2 h to 4 h. [Conclusion] At some concentrations the manganese was shown the cell-mediated immunotoxicity.

关 键 词：锰 细胞免疫功能 细胞免疫毒性作用 正交试验设计 

分 类 号：R992[医药卫生—毒理学] R995[医药卫生—药学]