6-[N-(4-氨基丁基)-N-乙基]氨基-2,3-二氢-1,4-酞嗪二酮的合成  

Synthesis of 6-[N-(4-aminobutyl)-N-ethyl]amino-2,3-dihydro-1,4-phthalazinedione

作　　者：匡永清[1] 孙晓莉[1] 何炜[1] 尉琳琳[1] 

机构地区：[1]第四军医大学基础部化学教研室,陕西西安710033

出　　处：《第四军医大学学报》2002年第11期1037-1039,共3页Journal of the Fourth Military Medical University

摘　　要：目的　建立一种合成化学发光标记试剂 6 - [N- (4-氨基丁基 ) - N-乙基 ]氨基 - 2 ,3-二氢 - 1,4 -酞嗪二酮 (ABEI)的简便、经济的新方法 .方法　室温下用 KOH将邻苯二甲酰亚胺转化为邻苯二甲酰亚胺钾盐 ,并进一步进行甲基化、硝化和还原反应 ,得到 N-甲基 - 4 -氨基邻苯二甲酰亚胺 .N- (4-溴丁基 )邻苯二甲酰亚胺与 N -甲基 - 4 -氨基邻苯二甲酰亚胺缩合之后 ,通过乙基化和肼解反应得到目的产物 ABEI.结果　按起始原料计算 ,ABEI的总收率为 4 .4 % .经熔点、核磁、质谱、红外、元素分析等方法鉴定 ,各中间体及目的产物均与其分子结构相符 .结论　起始原料价廉易得 ,反应步骤操作简便 。AIM To develop a simple and economical new approach to the synthesis of 6 [ N (4 aminobutyl) N ethyl] amino 2,3 dihydro 1,4 phthalazinedione (ABEI). METHODS Phthalimide was treated with KOH at room temperature to form potassium phthalamide, which was converted to N methyl 4 aminophthalimide by methylation, nitration and reduction reaction. Condensation of N (4 bro mobutyl) phthalimde with N methyl 4 aminophthalimide gave N methyl 4 N [4 ( N phthalimido)butyl]aminophthalimide, which was transformed into ABEI upon ethylation and hydrazinolysis. RESULTS The total yield of ABEI (calculated from the starting material phthalimide) was 4.4%. The intermediates and ABEI were characterized by melting point, 1H NMR, MS, IR and elemental analysis, and were found to be in agreement with the corresponding molecular structures. CONCLUSION The starting material of this new approach is of low price and easily available. The synthetic manipulations are simple and convenient, beneficial to reducing the cost of the product.

关 键 词：6-[N-(4-氨基丁基)-N-乙基]氨基-2 3-二氢-1 4-酞嗪二酮 合成 邻苯二甲酰亚胺 异鲁米诺衍生物 化学发光 化学发光免疫分析 

分 类 号：TQ463.24[化学工程—制药化工]