心肌缺血-再灌注时细胞凋亡及相关基因调控  

Regulation on the Apoptosis and Related Gene Expression Induced by Myocardial Ischemia-reperfusion

作　　者：冯津萍[1] 赵炳让[1] 卢奕[2] 姚智[2] 陈书中[3] 梁伯平[1] 梁爽霖[1] 

机构地区：[1]天津市胸科医院,天津300051 [2]天津医科大学,天津300070 [3]天津市塘沽医院,天津300450

出　　处：《天津中医》2002年第3期38-40,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：天津市卫生局科学基金资助 (98KYGG -8)

摘　　要：目的 :观察黄芪抑制家兔心肌缺血—再灌注时细胞凋亡的作用。方法 :采用结扎冠状动脉左前降支后再通的方法 ,复制家兔心肌缺血再灌注的动物模型 ,对心肌缺血—再灌注、黄芪治疗、心肌缺血、假手术等不同情况下心肌坏死面积、心肌细胞DNA电泳、凋亡细胞的形态 (TUNEL法、电镜 )及bcl-2与bax原位杂交进行了观察。结果 :再灌注组和黄芪治疗组的梗死面积小于单纯缺血组 ;DNA电泳发现再灌注组呈凋亡的典型表现 ,单纯缺血组表现为细胞坏死 ,黄芪治疗组梯形条带中DNA含量较心肌缺血—再灌注组明显减少 ;TUNEL染色发现缺血组有散在阳性细胞 ,再灌注组有大量的阳性标记 ,且积聚成团 ,黄芪治疗组较再灌注组明显减少 ;电镜观察发现再灌注组线粒体等细胞亚结构损伤严重 ,而黄芪治疗组则明显减轻。缺血组bcl-2与bax表达增多 ,再灌注组bax表达增多而bcl-2表达减少 ,黄芪治疗可下调bax且一定程度上调bcl-2。结论 :缺血再灌注可引起心肌细胞的凋亡 ,黄芪可确实抑制凋亡的发生。Objective: To observe the inhibitory effect of Radix Astragali (Huangqi)on apoptosis induced by myocardial ischemia-reperfusion (I/R). Method: By means of ligature and then repatency of left anterior descending branches coronary artery, the rabbit models with myocardial I/R were established. Myocardial DNA electrophoresis, myocardial apoptosis morphology (TUNEL), ultrastructural organization and in situ hybridization of bcl-2 and bax were observed in different situations. Results: The area of myocardial infarction in ischemia group was larger than that in Radix Astragali group and I/R group. The discovery of DNA electrophoresis in I/R group showed a typical apoptosis. The content of DNA in Radix Astragali group was lower than that in I/R group. The TUNEL staining found a few interspersed apoptosis cells in ischemia group, and the sum of apoptosis cells in I/R group was larger than that in Radix Astragali group. The mitochondria and other organellae injury in the I/R group was more serious than that in Radix Astragali group. The expressions of bcl-2 and bax in ischemia group were increased. The expression of bax was increased, but the expression of bcl-2 was decreased in the I/R group. The expression of bax was down regulated and the expression of bcl-2 was up regulated in Radix Astragali group. Conclusion: The myocardial apoptosis can be induced by I/R and ameliovated by Radix Astragali.

关 键 词：心肌缺血 再灌注 细胞凋亡 相关基因调控 黄芪 再灌注 BCL-2 BAX 

分 类 号：R542.2[医药卫生—心血管疾病] R285.5[医药卫生—内科学]