带有特定染色体片段的mini-F质粒所发动的染色体复制对于recA基因的依赖性  

recA Gene Dependence of Chromosome Replication of Escheric- hia coli Initiated by mini-F Plasmids Integrated at Predetermined Sites

作　　者：毛裕民[1] 盛祖嘉[1] 

机构地区：[1]复旦大学遗传学研究所,上海200433

出　　处：《Acta Genetica Sinica》1991年第1期73-81,共9页

基　　金：国家自然科学基金(3870279)

摘　　要：构建了一些带有大肠杆菌的特定染色体片段的mini-F质粒,使它们通过同源重组整合在dnaA46细菌的染色体的预定位置上,然后测定整合抑制菌株(sin)在40℃中染色体复制对recA基因的依赖性。实验结果说明,Sin菌株对recA基因的依赖性决定在质粒的整合位置。整合在oriC近旁的Sin菌株不依赖于recA基因;整合在oriC和terC中间的只在丰富培养基上是依赖的;整合在rerC附近的在不丰富的培养基上也依赖于recA基因。mini-F plasmids carrying particular chromosomal fragments were constructed. Through homologous recombination they were expected to integrate onto predetermined sites of the chromosome of dnaA46 strain LC381. Chromosome replication of suppressive integration (Sin) strains were recA dependent or independent according to the site of integration of the plasmid: chromosome replication of those with the plasmid integrated close to oriC were found to be recA dependent at 40℃; those with the plasmid integrated between oriC and terC were recA dependent on rich medium but independent on minimal medium; those with the plasmid integrated close to terC were recA dependent even on minimal medium.

关 键 词：mini-F质粒 整合抑制 RECA基因 

分 类 号：Q75[生物学—分子生物学]