HPLC测定骨愈宁胶囊中柚皮苷的含量  

DETERMINATION OF NARINGIN IN GUYULIN CAPSULES BY HPLC

作　　者：罗雪芹[1] 秦秀蓉[1] 吴懋芳[1] 

机构地区：[1]四川省中药研究所,四川成都610051

出　　处：《华西药学杂志》2002年第3期217-218,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的　测定骨愈宁胶囊中柚皮苷的含量。方法　采用高效液相色谱法 ,ShimpackCLC -ODS色谱柱 (15 0mm× 6 .0mm ,id ,5 μm) ,流动相为甲醇 - 36 %醋酸 -水 (36 :4:6 5 ) ,检测波长 2 83nm。 结果　柚皮苷在 0 .0 6 5～ 1.0 4μg范围内线性关系良好 (r =0 .9999) ,平均加样回收率为 10 0 .1% ,RSD =1.45 %。结论　该方法简便、准确。OBJECTIVE To develop an HPLC method for determination of naringin in Guyulin capsules.METHODS We used ODS column as stationary phase,methanol-36%acetic acid-water(36∶4∶65) as mobile phase,284 nm as detective wavelength.RESULTS The linerarity was good within the range of 0.065～1.04 μg(r =0.9999).The average recovery was 100.1% and RSD was 1.45%.CONCLUSION The method is simple,precise and available.

关 键 词：骨愈宁胶囊 柚皮苷 含量测定 大孔吸附树脂 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]