新的人趋化素样因子对骨髓造血干/祖细胞的体外刺激作用  

Effects of novel human chemokine-like factor 1 (CKLF1) on bone marrow hematopoietic stem cell/progenitor cell in vitro

作　　者：克晓燕[1] 贾丽萍[1] 景红梅[1] 刘彦[1] 张颖妹[2] 狄春晖[2] 

机构地区：[1]北京大学第三医院血液科,100083 [2]北京大学基础医学院免疫系

出　　处：《中华血液学杂志》2002年第6期301-303,共3页Chinese Journal of Hematology

摘　　要：目的　探讨新克隆的人趋化素样因子 (chemokine likefactor 1,CKLF1)对人造血干 /祖细胞的体外刺激作用。方法　分离正常人骨髓单个核细胞 ,进行液体和半固体集落培养 ,研究CKLF1对CFU GM和CFU Mix的作用。结果　加入转染CKLF1质粒上清组的CFU GM集落数明显增多 ,平均值为每 10 5细胞 2 34.81± 98.71,为对照组的 2 .4 2倍 (P <0 .0 5 ) ;CFU Mix平均集落数在正常对照、CKLF1、PHA和GM CSF组分别为每 10 5细胞 82 .0 0± 2 0 .2 5 ,10 5 .76± 36 .70 ,14 6 .6 3± 2 7.0 9和 14 3.33± 6 0 2 3,CKLF1的集落数多且较大 ,但统计学上差异无显著性 ;流式细胞术检测CD3 4 + 细胞百分率 ,正常对照、CKLF1、PHA、GM CSF组分别为 (0 .75± 0 .6 2 ) % ,(1.32± 0 .87) % ,(0 .15± 0 .0 2 ) % ,(0 .2 9± 0 .2 3) % ,与对照组相比 ,各组间差异无显著性。结论　新发现的具有CC家族结构的人趋化素样因子CKLF1对骨髓CFU GM集落的形成具有促增殖作用 。Objective To explore the effects of a novel human chemokine like factor 1(CKLF1) on stem cell/ progenitor cells. Methods Human bone marrow mononuclear cells were separated by Ficoll (1.077 g/ml), and cultured in suspension and semisolid colony culture. The effects of CKLF1 on CFU GM and CFU Mix were observed. Results The number of CFU GM was significantly increased in 10 groups by addition of CKLF1 plasmid supernatant. The mean value was 234.81±98.71/1×10 5 cells, 2.42 fold of control group (P<0.05). The mean value of CFU Mix in groups of negative control, CKLF1, PHA, GM CSF and G CSF were 82.00±20.25, 105.76±36.70, 146.63±27.09, 143.33±60.23 per 1×10 5 cells, respectively, no statistical differences were seen between control and CKLF1 groups. The CD 34 + cells were detected by FACS. The average percentage in the groups of normal control, CKLF1, PHA and GM CSF were (0.75±0.62)%,(1.32±0.87)%, (0.15±0.02)%, and (0.29±0 23)%, respectively. Compared with control, no significant differences were seen between each group (P>0.05). Conclusion Novel chemokine like factor 1 can increase the proliferation of CFU GM, which indicated that CKLF1 could increase the proliferation of human bone marrow hematopoietic progenitor cells and colony formation.

关 键 词：人趋化素样因子 骨髓 造血干细胞 造血祖细胞 体外刺激作用 

分 类 号：R392[医药卫生—免疫学]