TMR结合转基因技术促血管生成的形态学实验  

Experimental study on angiogenesis and revascularization in myocardial channel by combining TMR with transgenic technology

作　　者：张晓东[1] 黄秉仁[2] 王冬[1] 李若凡[1] 程效东[1] 刘克[1] 曲彦明[1] 徐达传[3] 

机构地区：[1]中国医学科学院基础医学研究所,中国协和医科大学基础医学院解剖组胚学系,北京100005 [2]中国医学科学院基础医学研究所,中国协和医科大学基础医学院生化学系 [3]第一军医大学解剖学教研室

出　　处：《解剖学杂志》2002年第3期210-213,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金资助 项目(39870785)

摘　　要：目的:观察机械性透室壁心肌血管重建术（Transmyocardial　revascularization,TMR）管道内转入VEGF基因后血管再生与重建的形态学效果,并探索TMR结合转基因技术的新方法。方法:利用高速空心钻头在家兔左室前壁进行TMR,形成透室壁性心肌管道。实验组的心肌管道内注入编码VEGFcDNA质粒;对照组仅做单纯机械性TMR。动物于术后不同时间处死,利用组织切片染色法,对照观察两组TMR管道内血管再生与重建状况。结果:实验组管道内新生小血管的密度月数量较高,与对照组相比具有非常显著性差异（P<0.001）。结论:机械TMR管道内注入VEGcDNA质粒后能有效地促进其血管生成,机械TMR结合转基因技术促血管生成作用明显优于单纯机械性TMR。Objective:To observe the morphogenetic results of angiogenesis and revascularization in the myocardial channels injected with VEGFcDNA naked plasmids after drilling TMR,as to obtain a new method of TMR combined with trans-genie technology. Methods;Under direct vision, transmyocardial channels were made by machine drilling on the left ventricular wall in 48 rabbits. The myocardial channels of experimental group were injected with VEGFcDNA plasmids;Those of the control group were treated only with drilling TMR. The animals were sacrificed at intervals of 1,2,4 and 8 weeks after operation. The angiogenesis and revascularization in channels were observed under microscope. Results; The density and quantity of neovessels (NV) in myocardial channels of 2 groups revealed a high significant difference (after 2 weeks, 8.41±0.59 NV per 10×4 field in control group versus 12.46 ± 0.49 NV per 10×4 field in experimental group,P<0. 001;after 8 weeks,3.31±0.57 NV per 10×4 field in control group versus 6.13±0.24 NV per 10×4 field in experimental group,P<0.001). Conclusion:Angiogenesis and revascularization are effectively promoted by injecting VEGFcDNA plasmids into the myocardial channels made by drilling TMR. The effect of stimulating angiogenesis resulted from drilling TMR combined with transgenic technology is obviously better than that resulted from simple drilling TMR.

关 键 词：机械性透室壁心肌血管重建术 血管内皮生长因子 质粒 血管生成 形态学 实验研究 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]