采用微团培养技术探讨双酚A的体外发育毒性  

Study on the developmental toxicity of Bisphenol A by using micromass culture in vitro

作　　者：李勇[1] 龙鼎新 裴新荣[1] 

机构地区：[1]北京大学公共卫生学院,北京100083

出　　处：《卫生研究》2002年第3期178-179,183,共3页Journal of Hygiene Research

基　　金：国家"973"项目 (No .G1 9990 5590 4 );国家自然科学基金重点项目 (No.30 0 30 1 2 0 )

摘　　要：采用微团培养观察了不同浓度的双酚A(0～ 5 0mg L)对体外培养的Wistar大鼠胚胎肢芽细胞增殖和分化的影响 ,旨在了解双酚A的体外发育毒性特点及其可能的作用机制。结果显示 :双酚A对体外培养的Wistar大鼠胚胎肢芽细胞的增殖和分化均有抑制作用 ,表现为染毒组肢芽细胞集落形成数量减少 ,细胞毒性试验吸光度值下降 ,并呈现出明显的剂量 反应关系。双酚A对肢芽细胞的 5 0 %增殖抑制浓度 (IP50 )和5 0 %分化抑制浓度 (ID50 )分别为 38 74mg L和 2 7 93mg L ,IP50 ID50 =1 39。按照Renault等人推荐的体外致畸分类标准 ,双酚A属于阳性致畸物 。The micromass culture of Wistar rat embryo limb bud cells was used to investigate the characteristic of developmental toxicity of Bisphenol A (BPA) and its mechanism in vitro. The results showed that BPA inhibited both proliferation and differentiation of rat limb bud cells in vitro. The high level of BPA appeared to be cytotoxic to Wistar rat embryo limb bud cells in culture and inhibited the clone formation with dose response relationship. The concentration of BPA for IP 50 and ID 50 were 38 74mg/L and 27 93mg/L respectively. According to Renaults' teratogenic criteria, BPA belonged to a positive teratogen. And the data suggested that the specific inhibition of cell proliferation and differentiation might be one of the mechanisms of high level BPA.

关 键 词：微团培养 双酚A 体外发育毒性 肢芽细胞 

分 类 号：R994.6[医药卫生—毒理学]