利用细胞工程技术筛选小麦抗根腐病突变体的研究  

Studies of Screening Resistant to Mutant Helminthosporium sativum by Cell Engineering in Wheat

作　　者：郭丽娟[1] 姚庆筱[1] 胡启德 康绍兰[2] 董金皋[2] 张汀[2] 黄梧芳[2] 

机构地区：[1]中国科学院遗传研究所,北京100101 [2]河北农业大学

出　　处：《Acta Genetica Sinica》1991年第6期500-507,共8页

基　　金：国家自然科学基金

摘　　要：以小麦根腐病菌002号菌株产生的致病培养滤液为选择剂,选用春、冬小麦品种、品系以及杂种的花药和幼德进行离体培养,并结合物理诱变技术,已获得抗根腐病的植株,用根腐病菌分生孢子接种鉴定,再生植株50株中有12株抗病。The fungal filtrate prepared from the cultures of H. sativum train No. 002 was used as screening agent. The anther and young inflorecences from varieties, strains and hybrids of spring and winter wheat were in vitro cultured. The calli were treated with physical mutagens. Finally the mutants resisting to H. sativum were obtained. Out of 50 regenerated plants resisting to fungal filtrate, 12 plants were resistant when put them to inoculation test with conisiospore of H. sativum.

关 键 词：小麦 根腐病 抗病突变体 筛选 离体 

分 类 号：S512.103.2[农业科学—作物学]