HBsAg阳性乙型肝炎患者淋巴细胞染色体脆性部位研究  

A STUDY OF CHROMOSOMAL FRAGILE SITE IN THE PERIPHERAL LYMPHOCYTE OF THE HEPITITIS B PATIENTS WITH HBsAg POSITIVE

作　　者：曹耒蓉 董孝泳 张峰[1] 张超英[1] 丛书英[2] 

机构地区：[1]青岛医学院生物学教研室 [2]青岛市传染病医院

出　　处：《遗传与疾病》1991年第1期25-27,64,共3页

摘　　要：用低叶酸,低小牛血清和较高pH值培养方法,对20例HBsAg阳性乙肝患者和20例正常人外周血淋巴细胞染色体畸变率和脆性部位进行了研究。结果表明患者组染色体畸变率和脆性部位表达率均显著高于对照组(P<0.001)。其中C-fra3p14在两组中表达率均最高、且有显著性差异(P<0.001)。并结合脆性部位与原发性肝癌发生的关系进行了讨论。The chromosomal fragile sites in lymphocyte were studied in 20 hepatitis B patients with HBsAg positive and 20 normal people as controls by cell culture with low concentration of serum, folate and higher pH in the medium. The results showed that the aberration rate of chromosome in hepatitis B patients was significantly higher than that in normal people (P<0.001).The short arm of chromosomes in D. G B.C. group exhibited expansion and deep stain in 70% of the patients. The aberrations may be another cytogentical expression of gene amplification in these fragments, which may have the same nature of the cytogenetical expression of gene amplification of the double minute in the cancer cell of hepatocellular carcinoma.

关 键 词：HBSAG 乙型肝炎 染色体 脆性部位 

分 类 号：R512.620.2[医药卫生—内科学]