基因脉冲导入仪的研制  

Development of gene pulse transfection apparatus

作　　者：徐宝强[1] 袁其平[1] 童峥嵘[1] 王德会[1] 刘建民 吕联荣[1] 

机构地区：[1]天津理工学院光电信息与电子工程系,天津300191

出　　处：《天津工业大学学报》2002年第2期9-11,共3页Journal of Tiangong University

基　　金：国家自然科学基金项目 (60 0 71 0 2 8)

摘　　要：针对基因转移技术的发展 ,开发了 L N- 10 1基因脉冲导入仪 ,介绍了其组成基本原理及实现的方法 .这一仪器的输出脉冲电压最大值为 2 5 0 0 V,电流最大值为 16 0 A,其转染率是用化学方法所得不到的 .该仪器具有高效率、低毒性、普适性和可控性 ,能够对多种类型的细胞实行基因导入 .There are more and more attentions on the method of introducing foreign DNA gene into host cells via gene transfer.In this article,the basic principle and the realizing method of LN-101 gene pulse are introduced.The gene pulse transfection apparatus is capable of producing pulses up to 2 500 V with over 160 A.Achieved transfection efficiencies by them are unable to obtain by chemical methods.This apparatus can be used in many kinds of cells which has the advantage of high efficiency,low toxicity,popularity and controllability.

关 键 词：基因脉冲导入仪 电穿孔 生物技术 转染率 组成 原理 实现方法 细胞 

分 类 号：Q813[生物学—生物工程] TN784[电子电信—电路与系统]