磁共振单克隆抗体免疫靶向对比剂McAb-DMP的制备  

Preparation of dextran-magnetite particles coated with CL-3 monoclonal antibody as targeting contrast agent for MRI

作　　者：陈伟[1] 张绍祥[2] 丁仕义[1] 任建敏[3] 

机构地区：[1]第三军医大学附属西南医院放射科,重庆400038 [2]第三军医大学基础医学部解剖学教研室,重庆400038 [3]第三军医大学医学检验系分析化学教研室,重庆400038

出　　处：《第三军医大学学报》2002年第6期634-635,共2页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 3992 50 2 2 )

摘　　要：目的　探讨磁共振单克隆抗体靶向对比剂McAb DMP的制备方法。方法　以葡聚糖为载体 ,采用氧化法制备免疫靶向磁性微粒。结果　制备的磁性纳米微粒与单抗相比保持了较高的结合活性和免疫活性。结论　合成CEA单克隆抗体标记磁性微粒 ,保证了结合活性免疫活性 。Objective To explore a preparing method for MRI target contrast agent, dextran magnetite particles coated with CL 3 monoclonal antibody (McAb DMP). Methods With dextran as carrier, McAb DMP particles was prepared by periodate procedure. The immune activities of the prepared particles were determined with ELISA. Results McAb DMP particles had a higher binding activity with periodate sodium at an amount of substance ratio of 1∶2, and kept better immune activity compared with unbound McAb. Conclusion McAb DMP particles guarantee the binding and immune activity in compared with McAb, providing a basis for in vivo study on animals

关 键 词：磁共振 单克隆抗体 免疫靶向 对比剂 McAb-DMP 制备 葡聚糖 磁性微粒 

分 类 号：R392.11[医药卫生—免疫学] R445.2[医药卫生—基础医学]