青蒿琥酯钠对大鼠抗孕作用的研究(英文)  

Effect of sodium artesunate on pregnancy in rats

作　　者：娄小娥[1] 周慧君[2] 杨波[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院,杭州310016 [2]浙江大学药学院,杭州310031

出　　处：《中国现代应用药学》2002年第3期172-175,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 :研究青蒿琥酯钠 (SA)对大鼠的抗孕作用以及其对妊娠及假孕大鼠血清孕酮含量和蜕膜组织的影响 ,以探讨其终止妊娠的机理。方法 :采用放射免疫分析法 (RIA)测定血清孕酮含量 ;HE染色法观察SA对大鼠子宫内膜的影响 ;用人蜕膜细胞体外培养方法观察SA对蜕膜细胞的直接作用。结果 :SA 40mg·kg 1·5d可使妊娠和假孕大鼠血清孕酮水平显著下降 (P<0 .0 5 ) ,但假孕大鼠血清孕酮水平下降晚于妊娠大鼠 ;整体水平能损伤蜕膜组织乃至胎盘 ;对体外培养的人蜕膜细胞有直接杀伤作用 ,其LC50 为 2 5 .18± 3.49ml·L 1。结论 :青蒿琥酯钠能使血清孕酮含量下降并损伤蜕膜和胎盘而使胚胎坏死、吸收而终止妊娠。OBJECTIVE:To study the effect of sodium artesunate (SA) on content of progesterone and decidua of pregnant rats and investigate the efficacy and mechanism of SA for termination of early pregnancy. METHOD:Serum content of progesterone was measured with RIA. The effect of SA on the overy, decidua and fetus of pregnant rats were studied using histochemistry techniques. Decidual cells were estimated using cell culture. RESULTS:SA 40 mg·kg 1 ·d 1 given sc on d 6 10 of gestation significantly decreased the concentration of serum progesterone in early pregnant rats; decidual cells and fetus of treated groups were found to be degenerated at d 11. SA was shown to directly damage the decidual cells. Cultured human decidual cells were exposed to atesunate for 48h, LC 50 was found to be 25.18±3.49 ml·L 1 .CONCLUSIONS:SA had a feticide effect in rats and the damage on decidua and placenta may be the mechanisms of its contragestational action.

关 键 词：青蒿琥酯钠 大鼠 抗孕作用 研究 孕酮 蜕膜 终止妊娠 

分 类 号：R169.42[医药卫生—公共卫生与预防医学]