声波刺激对猕猴桃愈伤组织的生理效应  

Brief Study on Physiological Effects of Sound Field on Actinidia Chinensis Callus

作　　者：阳小成[1] 王伯初[1] 段传人[1] 戴传云[1] 贾毅[1] 王秀娟[1] 

机构地区：[1]重庆大学生物工程学院教育部生物力学与组织工程重点实验室,重庆400044

出　　处：《重庆大学学报（自然科学版）》2002年第4期79-83,共5页Journal of Chongqing University

基　　金：国家自然科学基金资助项目 (3 9770 2 0 6)

摘　　要：用强声波处理中华猕猴桃 (Actinidiachinensisplanch)茎段的愈伤组织 ,研究模拟声场的力学刺激对木本植物愈伤组织生长分化的生理生化效应 ,并就声波对植物发生作用的可能机理进行了探讨。结果表明 ,猕猴桃对强声波刺激的应激效应与本实验室此前对草本植物非洲雏菊 (GerberaJamesoniiacrocarpous)的研究有相似的结果。即适度频率和强度的声波刺激 (10 0 0Hz、10 0dB)可促进猕猴桃愈伤组织的生长 ,使胞内可溶性蛋白质含量升高 ,SOD活性升高 ,而IAA氧化酶活性降低 ;但当声波刺激超过一定范围后 ,愈伤组织的生长受到明显的抑制 ,可溶性蛋白质含量下降 ,SOD活性降低而IAA氧化酶活性升高。研究结果显示 :植物对外界强声波刺激的应激效应是很明显的。Insight into the relationship between plant cell (tissue) and physical stimulation is a focus of biomechanics. In this study, several important plant physiological indexes of Actinidia chinensis callus in sound field including the content of soluble proteins, the activity of SOD, the activity of IAA oxidase and the penetrability of cell membrane, were measured. Some information is got about stress effect through the study and try to explore its mechanism. From the result of experiment,we found that there were a great similarity of stress effect on sound field between Actinidia chinensis and Gerbera Jamesonii acrocarpous , a kind of herbage plant, which had been studied in our lab before. That is to say, the sound field also has dual effect on woody plant. It can enhance or inhibit the growth and development of Actinidia chinensis callus along with the sound field frequency ,and the moderate frequency of sound field acting on the callus could promote callus growth. A conclusion is drawn that the optimal stimulation of sound field are 100 dB and 1 000 Hz. Under the conditions, the sound field can distinctly enhance the growth of Actinidia chinensis callus Whereas, it is not very easy to explore the detail mechanism of environmental stress effects on plant. This paper discussed the mechanism of sound field stress on plant from the level of cell and molecule.

关 键 词：声波刺激 中华猕猴桃 愈伤组织 应激效应 

分 类 号：Q947[生物学—植物学]