钴卟啉修饰碳纤维束葡萄糖酶微电极的研究  

GLUCOSE MICRO-ENZYME SENSOR BASED ON COBALT PORPHYRIN MODIFIED MICRODISK ARRAY CARBON FIBER ELECTRODE

作　　者：冯连玉[1] 车广礼 董绍俊[1] 章咏华[1] 

机构地区：[1]中国科学院长春应用化学研究所电分析化学开放实验室,长春130022

出　　处：《应用化学》1991年第3期86-88,共3页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金

摘　　要：生物电化学传感器的微型化研究是目前生物传感器研究中的一个很活跃的方向。我们曾用钴卟啉修饰碳纤维柱电极,并以它为基底制成了葡萄糖微传感器。为加强电极的抗折断能力,本文把约1千支碳纤维(φ9μm)做成碳纤维束盘微电极,在其表面修饰四苯基钴卟啉后,以其为基底制成了葡萄糖酶电极。将其用于流动注射分析,可以大大提高线性响应上限。A cobalt tetraphenyl porphyrin modified microdisk array carbon fiber electrode hasbeen prepared which showed a catalytic activity for oxygen reduction through linear voltam-metry. A glucose microenzyme electrode has been made based on the modified microelectrodeby immobilizing glucose oxidase. The upper detection limit of the microsensor was2.4mmol/L. The response took 40s with satisfactory selectivity. The sensor held linear upto 13mmol/L in use for amperometric detection in the flow injection analysis.

关 键 词：葡萄糖 生物传感器 微电极 酶电极 

分 类 号：Q532.3[生物学—生物化学]