中药大腹皮抑制肠道内毒素移位中iNOS、SP的作用  

Inhibitory role of iNOS and SP in intestinal endotoxin translocation induced by pericarpium arecae in rats

作　　者：朱金照[1] 张捷[1] 许其增[1] 张志坚[1] 冷恩仁[2] 

机构地区：[1]中国人民解放军南京军区福州总医院消化内科,福建省福州市350025 [2]第三军医大学大坪医院消化内科,重庆市400042

出　　处：《世界华人消化杂志》2002年第6期659-662,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨大腹皮抑制肠道内毒素移位的可能机制,为其临床应用提供理论依据。 方法:wistar大鼠30只随机分为对照组,内毒素组及内毒素+大腹皮组,分别给对照组、内毒素组及内毒素+大腹皮组经硅胶管向十二指肠注入生理盐水,荧光内毒素及荧光内毒素+大腹皮水提液,3h后观察大鼠血浆荧光内毒素含量的变化,同时应用免疫组织化学法观察空、回肠一氧化氮合酶(iNOS)及P物质(SP)的分布变化。 结果:内毒素组血浆荧光内毒素的含量显著高于对照组(0.223±0.041 vs 0.087±0.016,P<0.01,内毒素+大腹皮组血浆荧光内毒素的含量明显低于内毒素组(0.135±0.028 vs 0.223±0.041,P<0.05),与对照组无明显差异。内毒素组空、回肠一氧化氮合酶的分布明显高于对照组(1.97±0.27 vs 3.37±0.31,2.23±0.38 vs 2..91±0.31,2.11±0.23 vs 3.42±0.38;1.81±0.32 vs 3.12±0.29,2.06±0.35 vs 2.75±0.26,2.08±0.20 vs3.38±0.31。P<0.05或P<0.01),P物质明显低于对照组(3.25±0.35 vs 2.81±0.21,3.12±0.31 vs 2.68±0.22,2.74±0.33vs 2.34±0.24;2.36±0.25 vs 1.95±0.22,3.16±0.37 vs 2.77±0.18,2.78±0.29 vs 2.37±0.17。P<0.05),内毒素+大腹皮组空、回肠一氧化氮合酶及P物质的分布与对照组无明显差异(P>0.05)。AIM: To study the mechnisms of pericarpium arecae in suppression of intestinal endotoxin translocation in rats.METHODS: Total 30 Wistar rats were randomly divided into control group, endotoxin group and endotoxin plus pericarpium arecae group. Saline, endotoxin and endotoxin plus decoction of pericarpium arecae were respectively poured into duodenum of rats by intubation. After 3 hours of administration, the level of endotoxin in plasma of rats were assayed. Meanwhile,the distributions of iNOS and SP in jejunum and ileum were investigated through immunohistochemistry.RESULTS: Compared with control group, the level of endotoxin in plasma in rats of endotoxin group was significantly increased (0.223 ±0.041 vs 0.087 ±0.016, P<0. 01). The level of endotoxin in plasma in the endotoxin plus pericarpium arecae group was significantly lower than that in endotoxin group (0.135 ± 0.028 vs 0.223 ± 0.041, P < 0.05). Expressions of iNOS increased and the SP decreased significantly in endotoxin group.CONCLUSION:The changes of iNOS and SP in small intestine play certain roles in intestinal endotoxin translocation, and pericarpium arecae can decrease intestinal endotoxin translocation by regulating the distributions of iNOS and SP in jejunum and ileum .

关 键 词：大腹皮 肠道内毒素移位 INOS P物质 免疫组织化学 内毒素血症 

分 类 号：R285[医药卫生—中药学]